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Literature summary for 3.6.1.11 extracted from

  • Luginbuehl, E.; Kunz, S.; Wentzinger, L.; Freimoser, F.; Seebeck, T.
    The exopolyphosphatase TbrPPX1 of Trypanosoma brucei (2011), BMC Microbiol., 11, 4-4.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.11

Activating Compound

Activating Compound Comment Organism Structure
additional information the activity of PPX1 is not affected by cAMP, deoxynucleoside triphosphates, ATP, sodium diphosphate, L-arginine, or by long polyanions such as heparin or RNA Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
additional information PPX1 is not inhibited by Ro-20-1724, sildenafil, zaprinast, papaverine or etazolate, or the sodium salts of vanadate, fluoride or sulfate Trypanosoma brucei
Zn2+ strong inhibitor Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0272
-
 pentasodium triphosphate in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Trypanosoma brucei 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42800
-
 SDS-PAGE Trypanosoma brucei
42870
-
 calculated from amino acid sequence Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q7Z032
-
-
Trypanosoma brucei 427 Q7Z032
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose resin column chromatography or amylose-resin column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O
-
 Trypanosoma brucei (polyphosphate)n-1 + phosphate
-
 ?
(polyphosphate)n + H2O
-
 Trypanosoma brucei 427 (polyphosphate)n-1 + phosphate
-
 ?
additional information the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity Trypanosoma brucei ?
-
 ?
additional information the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity Trypanosoma brucei 427 ?
-
 ?
pentasodium triphosphate + H2O
-
 Trypanosoma brucei pentasodium diphosphate + phosphate
-
 ?
pentasodium triphosphate + H2O
-
 Trypanosoma brucei 427 pentasodium diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PPX1
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.1
-
 pentasodium triphosphate in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma brucei calculated from amino acid sequence
-
 5.4

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0213
-
 in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei Zn2+

General Information

General Information Comment Organism
malfunction PPX1 genetic ablation does not produce a dramatic phenotype Trypanosoma brucei