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Literature summary for 3.6.1.11 extracted from

  • Tammenkoski, M.; Koivula, K.; Cusanelli, E.; Zollo, M.; Steegborn, C.; Baykov, A.A.; Lahti, R.
    Human metastasis regulator protein H-prune is a short-chain exopolyphosphatase (2008), Biochemistry, 47, 9707-9713.
    View publication on PubMed
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as His-tagged wild type protein and His-tagged variants Homo sapiens

Protein Variants

Protein Variants Comment Organism
D106A variant displays reduced activity with a turnover value of 35% compared to the wild type counterpart Homo sapiens
D179A variant is inactive Homo sapiens
D28A variant is inactive Homo sapiens
H107N variant displays reduced activity with a turnover value of 4.4% compared to the wild type counterpart, Km value increases 7fold Homo sapiens
H108N variant displays reduced activity with a turnover value of 32% compared to the wild type counterpart Homo sapiens
N24H variant is inactive Homo sapiens
R128H enhanced kcat value (146%) is obtained with the mutant protein compared to the wild type counterpart, Km value increases 21fold Homo sapiens
R348A variant is inactive Homo sapiens

General Stability

General Stability Organism
Mg2+ stabilizes h-prune against inactivation during storage Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(phosphate)25
-
 Homo sapiens
(phosphate)45
-
 Homo sapiens
(phosphate)65
-
 Homo sapiens
diphosphate potential physiological regulator Homo sapiens
dipyridamole known inhibitor of phosphodiesterase, no effect on the triphosphate hydrolyzing activity of h-prune Homo sapiens
long-chain polyphosphate potential physiological regulator, inhibits h-prune-catalyzed hydrolysis of triphosphate Homo sapiens
additional information nucleoside triphosphates, diadenosine hexaphosphate, cAMP and dipyridamole do not affect the activity Homo sapiens
nm23-H1 metastasis suppressor protein, the exopolyphosphatase activity is suppressed Homo sapiens
ZnCl2
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 (phosphate)65 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0012
-
 (phosphate)3 D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0022
-
 (phosphate)3 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0022
-
 (phosphate)45 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0026
-
 (phosphate)3 H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0066
-
 (phosphate)3 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.0069
-
 (phosphate)25 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.015
-
 (phosphate)3 H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.019
-
 (phosphate)4 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.028
-
 adenosine 5'-tetraphosphate 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.037
-
 adenosine 5'-tetraphosphate 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.041
-
 (phosphate)4 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.041
-
 guanosine 5'-tetraphosphate 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.046
-
 (phosphate)3 R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.099
-
 guanosine 5'-tetraphosphate 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reaction requires a divalent metal cofactor Homo sapiens
Mg2+ reaction requires a divalent metal cofactor, bound substrate enhances enzyme affinity for the metal ion Homo sapiens
Mn2+ reaction requires a divalent metal cofactor, Mn2+ confers 50% activity compared to Mg2+ in P3 and P4 hydrolysis Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 estimated from the amino acid composition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(phosphate)n + H2O Homo sapiens h-prune efficiently hydrolyzes short-chain polyphosphates (phosphate)n-1 + phosphate
-
 ?
additional information Homo sapiens h-prune is the missing exopolyphosphatase in animals and support the hypothesis that the metastatic effects of h-prune are modulated by inorganic polyphosphates, which are increasingly recognized as critical regulators in cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by metal chelate chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 h-prune efficiently hydrolyzes short-chain polyphosphates, including inorganic tripoly- and tetrapolyphosphates and nucleoside 5'-tetraphosphates, long-chain inorganic polyphosphates (more than 25 phosphate residues) are converted more slowly Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)25 + H2O
-
 Homo sapiens (phosphate)24 + phosphate
-
 ?
(phosphate)4 + H2O
-
 Homo sapiens (phosphate)3 + phosphate
-
 ?
(phosphate)45 + H2O
-
 Homo sapiens (phosphate)44 + phosphate
-
 ?
(phosphate)65 + H2O
-
 Homo sapiens (phosphate)64 + phosphate
-
 ?
(phosphate)n + H2O h-prune efficiently hydrolyzes short-chain polyphosphates Homo sapiens (phosphate)n-1 + phosphate
-
 ?
adenosine 5'-tetraphosphate + H2O
-
 Homo sapiens ATP + phosphate
-
 ?
guanosine 5'-tetraphosphate + H2O
-
 Homo sapiens GTP + phosphate
-
 ?
additional information h-prune is the missing exopolyphosphatase in animals and support the hypothesis that the metastatic effects of h-prune are modulated by inorganic polyphosphates, which are increasingly recognized as critical regulators in cells Homo sapiens ?
-
 ?
triphosphate + H2O
-
 Homo sapiens diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
exopolyphosphatase
-
 Homo sapiens
h-prune
-
 Homo sapiens
PPX
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 incubation of h-prune 1-100 microM for 5 h in the presence of 0.1 M Tris-HCl, pH 7.2, and 0.05 mM EGTA inactivates the enzyme 2-4fold, no inactivation is evident in the presence of 1 mM Mg2+ Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 (phosphate)65 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.16
-
 (phosphate)25 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.22
-
 (phosphate)45 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
0.57
-
 (phosphate)3 H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
3.4
-
 (phosphate)4 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
4.2
-
 (phosphate)3 H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
4.6
-
 (phosphate)3 D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
7.1
-
 (phosphate)4 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
13
-
 (phosphate)3 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
19
-
 (phosphate)3 R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
24
-
 guanosine 5'-tetraphosphate 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
27
-
 guanosine 5'-tetraphosphate 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
31
-
 adenosine 5'-tetraphosphate 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
35
-
 (phosphate)3 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens
39
-
 adenosine 5'-tetraphosphate 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 2 mM Mg2+, 60 microM (phosphate)25, 10 mM triphosphate, IC50 is above 0.01 mM Homo sapiens nm23-H1
0.0019
-
 2 mM Mg2+, 10 mM triphosphate Homo sapiens nm23-H1
0.0072
-
 2 mM Mg2+, 10 mM triphosphate Homo sapiens nm23-H1
0.032
-
 2 mM Mg2+, hydrolysis of triphospate is inhibited Homo sapiens ZnCl2
0.055
-
 2 mM Mg2+, 10 mM triphosphate Homo sapiens (phosphate)45
0.064
-
 c2 mM Mg2+, 10 mM triphosphate Homo sapiens (phosphate)65
0.105
-
 c2 mM Mg2+, 100 mM triphosphate Homo sapiens (phosphate)65
0.12
-
 2 mM Mg2+, 100 mM triphosphate Homo sapiens (phosphate)45
0.163
-
 2 mM Mg2+, 10 mM triphosphate Homo sapiens (phosphate)25
0.33
-
 2 mM Mg2+, 100 mM triphosphate Homo sapiens (phosphate)25
1.2
-
 2 mM Mg2+, 10 mM triphosphate Homo sapiens diphosphate
5.7
-
 2 mM Mg2+, 100 mM triphosphate Homo sapiens diphosphate