Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Saccharomyces cerevisiae | |
heparin | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell envelope | - |
Saccharomyces cerevisiae | 30313 | - |
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
cytosol | appears in the cytosol under PPX1 inactivation | Saccharomyces cerevisiae | 5829 | - |
mitochondrial membrane | - |
Saccharomyces cerevisiae | 31966 | - |
mitochondrion | soluble fraction | Saccharomyces cerevisiae | 5739 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
determined by gel filtration | Saccharomyces cerevisiae |
450000 | - |
determined by gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | PPN1 splitting long polyphosphate chains to shorter ones | ? | - |
? | |
additional information | Saccharomyces cerevisiae | PPX1 splitting off phosphate from the end of the polyphosphate chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
polyphosphate hydrolysis in the CRX strain cytosol completes in 120 min | Saccharomyces cerevisiae |
0.006 | - |
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium | Saccharomyces cerevisiae |
0.01 | - |
strain CRX with inactivated ppx1 and ppN1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium | Saccharomyces cerevisiae |
0.04 | - |
strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium | Saccharomyces cerevisiae |
0.055 | - |
strain CRX with inactivated ppx1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium | Saccharomyces cerevisiae |
0.07 | - |
strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-rich medium | Saccharomyces cerevisiae |
0.095 | - |
strain CRX with inactivated ppn1 gene, cytosol preparation in exponential growth phase in phosphate-deficient medium | Saccharomyces cerevisiae |
0.1 | - |
parent strain CRY, cytosol preparation in exponential growth phase in phosphate-deficient medium | Saccharomyces cerevisiae |
0.18 | - |
parent strain CRY, cytosol preparation in exponential growth phase in phosphate-rich medium | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PPN1 splitting long polyphosphate chains to shorter ones | Saccharomyces cerevisiae | ? | - |
? | |
additional information | PPX1 splitting off phosphate from the end of the polyphosphate chain | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exopolyphosphatase | - |
Saccharomyces cerevisiae |
Ppn1 | - |
Saccharomyces cerevisiae |
PPX1 | - |
Saccharomyces cerevisiae |