Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of PPX results in a dramatic decrease in total short-chain polyphoaphates and partial decrease in long-chain polyphosphates accompanied by a delayed regulatory volume decrease after hyposmotic stress, overview | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | inhibition of the recombinant enzyme at high concentrations | Trypanosoma cruzi | |
heparin | inhibition of the recombinant enzyme at high concentrations | Trypanosoma cruzi | |
KCl | slight inhibition at high concentrations | Trypanosoma cruzi | |
lysine | inhibition of the recombinant enzyme at high concentrations | Trypanosoma cruzi | |
NaCl | slight inhibition at high concentrations | Trypanosoma cruzi | |
Zn2+ | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
guanosine 5'-tetraphosphate | pH 7.5, 30°C | Trypanosoma cruzi | |
0.058 | - |
Triphosphate | pH 7.5, 30°C | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Trypanosoma cruzi | |
Mg2+ | activates, preferred divalent cation | Trypanosoma cruzi | |
Mn2+ | activates | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma cruzi | polyphosphates play a role in the parasite's osmoregulation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q6Y656 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine 5'-tetraphosphate + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
additional information | polyphosphates play a role in the parasite's osmoregulation, overview | Trypanosoma cruzi | ? | - |
? | |
additional information | the enzyme prefers short-chain polyphosphates, substrate specificity, TcPPX is a processive enzyme and does not hydrolyze ATP, diphosphate, or 4-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently, overview | Trypanosoma cruzi | ? | - |
? | |
triphosphate + H2O | - |
Trypanosoma cruzi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPX | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
guanosine 5'-tetraphosphate | pH 7.5, 30°C | Trypanosoma cruzi | |
3.46 | - |
Triphosphate | pH 7.5, 30°C | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |