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Literature summary for 3.6.1.11 extracted from

  • Fang, J.; Ruiz, F.A.; Docampo, M.; Luo, S.; Rodrigues, J.C.; Motta, L.S.; Rohloff, P.; Docampo, R.
    Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation (2007), J. Biol. Chem., 282, 32501-32510.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
additional information overexpression of PPX results in a dramatic decrease in total short-chain polyphoaphates and partial decrease in long-chain polyphosphates accompanied by a delayed regulatory volume decrease after hyposmotic stress, overview Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
arginine inhibition of the recombinant enzyme at high concentrations Trypanosoma cruzi
heparin inhibition of the recombinant enzyme at high concentrations Trypanosoma cruzi
KCl slight inhibition at high concentrations Trypanosoma cruzi
lysine inhibition of the recombinant enzyme at high concentrations Trypanosoma cruzi
NaCl slight inhibition at high concentrations Trypanosoma cruzi
Zn2+
-
Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
guanosine 5'-tetraphosphate pH 7.5, 30°C Trypanosoma cruzi
0.058
-
Triphosphate pH 7.5, 30°C Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Trypanosoma cruzi
Mg2+ activates, preferred divalent cation Trypanosoma cruzi
Mn2+ activates Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma cruzi polyphosphates play a role in the parasite's osmoregulation, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q6Y656
-
-

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
Trypanosoma cruzi
-
epimastigote
-
Trypanosoma cruzi
-
trypomastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 5'-tetraphosphate + H2O
-
Trypanosoma cruzi ?
-
?
additional information polyphosphates play a role in the parasite's osmoregulation, overview Trypanosoma cruzi ?
-
?
additional information the enzyme prefers short-chain polyphosphates, substrate specificity, TcPPX is a processive enzyme and does not hydrolyze ATP, diphosphate, or 4-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently, overview Trypanosoma cruzi ?
-
?
triphosphate + H2O
-
Trypanosoma cruzi ?
-
?

Synonyms

Synonyms Comment Organism
PPX
-
Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
guanosine 5'-tetraphosphate pH 7.5, 30°C Trypanosoma cruzi
3.46
-
Triphosphate pH 7.5, 30°C Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Trypanosoma cruzi