Any feedback?
Literature summary for 3.6.1.11 extracted from
- Purification and properties of exopolyphosphatase from the cytosol of Saccharomyces cerevisiae not encoded by the PPX1 gene (2004), Biochemistry, 69, 387-393.
General Stability
| General Stability | Organism |
|---|---|
| addition of 0.05 mM CoSO4 does not increase the enzyme stability independent of the presence of Triton X-100 | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 1 mM, 24% activity loss; 25% inactivation at 1 mM | Saccharomyces cerevisiae |  |
| dithiothreitol | 0.1 mM, 13% activity loss | Saccharomyces cerevisiae | |
| EDTA | 0.1 mM, 100% activity loss; complete inactivation at 0.1 mM | Saccharomyces cerevisiae | |
| heparin | 1 mg/l 65% activity loss, 10 mg/l 95% activity loss; 95% inactivation at 0.01 mg /ml | Saccharomyces cerevisiae | |
| iodoacetamide | 10 mM, 26% activity loss; 25% inactivation at 10 mM | Saccharomyces cerevisiae | |
| additional information | not inhibited by antibodies suppressing the activity of 40 kDa-exopolyphosphatase | Saccharomyces cerevisiae | |
| NaF | 10 mM, 9% activity loss | Saccharomyces cerevisiae | |
| polyphosphate 3 | 1mM, 4% activity loss | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
(Polyphosphate)n | n = 208, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 0.075 | - |
(Polyphosphate)n | n = 15, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 1.1 | - |
(Polyphosphate)n | n = 3, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 3.5 | - |
polyphosphate 208 | - |
Saccharomyces cerevisiae | |
| 75 | - |
polyphosphate 15 | - |
Saccharomyces cerevisiae | |
| 1100 | - |
polyphosphate 3 | - |
Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 6fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| Co2+ | 0.1 mM, 6fold activity stimulation | Saccharomyces cerevisiae | |
| KCl | 50 and 200 mM, 39 and 38% activity enhancement, respectively | Saccharomyces cerevisiae | |
| Mg2+ | 2fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| Mg2+ | 0.1 mM MgSO4, 5% activity loss | Saccharomyces cerevisiae | |
| Mg2+ | 1 mM, 2fold activity enhancement | Saccharomyces cerevisiae | |
| additional information | stimulated by divalent cations to a lesser extent | Saccharomyces cerevisiae | |
| NaCl | 50 and 200 mM, 46 and 42% activity enhancement, respectively | Saccharomyces cerevisiae | |
| NH4Cl | 50 and 200 mM, 42 and 67% activity enhancement, respectively | Saccharomyces cerevisiae | |
| Zn2+ | 1.5fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| Zn2+ | 0.1 mM ZnSO4, 76% activity loss | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
| 35000 | - |
x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
| 125000 | - |
Superose 6 column gel filtration, active but unstable enzyme complex | Saccharomyces cerevisiae |
| 500000 | - |
Superose 6 column gel filtration, stable enzyme complex composed of two polypeptides of 32 and 35 kDa and apparently polyphosphates | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae VKM Y-1173 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl 650 M column chromatography | Saccharomyces cerevisiae |
| partial, using ion-exchange chromatography and gel filtration | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (polyphosphate)n + H2O = (polyphosphate)n-1 + phosphate | hydrolyzes poly-phosphates with an average chain length of 208 to 15 phosphate residues | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no specific activity on diphosphate, ATP and p-nitrophenyl phosphate | Saccharomyces cerevisiae |
| 0.47 | - |
enzyme from cystosol | Saccharomyces cerevisiae |
| 1.11 | - |
after ammonium sulfate precipitation | Saccharomyces cerevisiae |
| 5 | - |
after DEAE-Toyopearl 650 M column chromatography | Saccharomyces cerevisiae |
| 150 | - |
pH 7.2, 30°C, polyP208 as substrate | Saccharomyces cerevisiae |
| 150 | - |
after 319fold purification with heparin agarose, at 30°C in 1 ml of reaction mixture containing 50 mM Tris-HCl buffer, pH 7.2, 0.1 mM CoSO4, 200 mM ammonium chloride, and 0.01 mM polyphosphate 208 | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -4°C, 0.1% Triton X-100, 1M KCl, 4 days, no activity loss | Saccharomyces cerevisiae |
| -4°C, enzyme desorbed from heparin-agarose with 1 M KCl and 0.1% Triton X-100, 4 days, no loss of activity | Saccharomyces cerevisiae |
| 4°C, enzyme obtained by desorption with polyphosphate, 2 h, 30% loss of activity | Saccharomyces cerevisiae |
| 92°C, in the presence of 0.1% Triton X-100, 5 days, 8% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography | Saccharomyces cerevisiae |
| 92°C, without 0.1% Triton X-100, 5 days, 66% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae | (polyphosphate)n-1 + phosphate | - |
? | |
| (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | (polyphosphate)n-1 + phosphate | - |
? | |
| additional information | ATP, diphosphate and p-nitrophenyl phosphate are not substrates | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | ATP, diphosphate and p-nitrophenyl phosphate are not substrates | Saccharomyces cerevisiae VKM Y-1173 | ? | - |
? | |
| polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 14 + phosphate | - |
? | |
| polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 14 + phosphate | - |
? | |
| polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 207 + phosphate | - |
? | |
| polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 207 + phosphate | - |
? | |
| polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 2 + phosphate | - |
? | |
| polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 2 + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| high molecular mass exopolyphosphatase | - |
Saccharomyces cerevisiae |
| polyphosphate phosphohydrolase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
