Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.11 extracted from

  • Lorenz, B.; Mueller, W.E.G.; Kualev, I.S.; Schroeder, H.C.
    Purification and characterization of an exopolyphosphatase from Saccharomyces cerevisiae (1994), J. Biol. Chem., 269, 22198-22204.
    View publication on PubMed

General Stability

General Stability Organism
repeated freezing and thawing destroys activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ competitive with respect to Mg2+ Saccharomyces cerevisiae
Cu2+
-
Saccharomyces cerevisiae
diphosphate competitive Saccharomyces cerevisiae
NaCl 400 mM, about 41% loss of activity Saccharomyces cerevisiae
o-vanadate 10 mM, nearly complete inhibition Saccharomyces cerevisiae
phosphate no effect Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
polyP33-36
-
Saccharomyces cerevisiae
0.033
-
polyP9-10
-
Saccharomyces cerevisiae
0.41
-
tripolyphosphate
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 108% of the activation by Mg2+ Saccharomyces cerevisiae
Fe2+ 5 mM, 34% of the activation by Mg2+ Saccharomyces cerevisiae
Mg2+ required, optimal activity at 5 mM Saccharomyces cerevisiae
Ni2+ 5 mM, 32% of the activation by Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl buffer, pH 7.6, 10 mM MgCl2, 0.5 mM EDTA, 150 mM NaCl, 20% loss of activity after 2 months Saccharomyces cerevisiae
4°C, in absence of detergents, complete loss of activity after 24 h, can be stabilized by 0.1% Triton X-100 and protease inhibitors Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O poly P9-10, polyP33-36 Saccharomyces cerevisiae (polyphosphate)n-1 + phosphate
-
?
polyP33-36
-
Saccharomyces cerevisiae polyP32-35 + phosphate
-
?
polyP9-10
-
Saccharomyces cerevisiae polyP8-9 + phosphate
-
?
tripolyphosphate + H2O
-
Saccharomyces cerevisiae phosphate + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
81
-
polyP33-36
-
Saccharomyces cerevisiae
90
-
polyP9-10
-
Saccharomyces cerevisiae
143
-
tripolyphosphate
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 9 45-55% of maximal activity at pH 6.0 and at pH 9.0 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
4°C, 48 h, 95% loss of activity Saccharomyces cerevisiae
5
-
4°C, 48 h, 66% loss of activity Saccharomyces cerevisiae
6
-
4°C, 48 h, 21% loss of activity Saccharomyces cerevisiae
7
-
4°C, 48 h, 5% loss of activity Saccharomyces cerevisiae
7.5 8 4°C, 48 h, stable Saccharomyces cerevisiae
9
-
4°C, 48 h, 11% loss of activity Saccharomyces cerevisiae
10
-
4°C, 48 h, 95% loss of activity Saccharomyces cerevisiae