General Stability | Organism |
---|---|
repeated freezing and thawing destroys activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | competitive with respect to Mg2+ | Saccharomyces cerevisiae | |
Cu2+ | - |
Saccharomyces cerevisiae | |
diphosphate | competitive | Saccharomyces cerevisiae | |
NaCl | 400 mM, about 41% loss of activity | Saccharomyces cerevisiae | |
o-vanadate | 10 mM, nearly complete inhibition | Saccharomyces cerevisiae | |
phosphate | no effect | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
polyP33-36 | - |
Saccharomyces cerevisiae | |
0.033 | - |
polyP9-10 | - |
Saccharomyces cerevisiae | |
0.41 | - |
tripolyphosphate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 108% of the activation by Mg2+ | Saccharomyces cerevisiae | |
Fe2+ | 5 mM, 34% of the activation by Mg2+ | Saccharomyces cerevisiae | |
Mg2+ | required, optimal activity at 5 mM | Saccharomyces cerevisiae | |
Ni2+ | 5 mM, 32% of the activation by Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl buffer, pH 7.6, 10 mM MgCl2, 0.5 mM EDTA, 150 mM NaCl, 20% loss of activity after 2 months | Saccharomyces cerevisiae |
4°C, in absence of detergents, complete loss of activity after 24 h, can be stabilized by 0.1% Triton X-100 and protease inhibitors | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(polyphosphate)n + H2O | poly P9-10, polyP33-36 | Saccharomyces cerevisiae | (polyphosphate)n-1 + phosphate | - |
? | |
polyP33-36 | - |
Saccharomyces cerevisiae | polyP32-35 + phosphate | - |
? | |
polyP9-10 | - |
Saccharomyces cerevisiae | polyP8-9 + phosphate | - |
? | |
tripolyphosphate + H2O | - |
Saccharomyces cerevisiae | phosphate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81 | - |
polyP33-36 | - |
Saccharomyces cerevisiae | |
90 | - |
polyP9-10 | - |
Saccharomyces cerevisiae | |
143 | - |
tripolyphosphate | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 45-55% of maximal activity at pH 6.0 and at pH 9.0 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
4°C, 48 h, 95% loss of activity | Saccharomyces cerevisiae |
5 | - |
4°C, 48 h, 66% loss of activity | Saccharomyces cerevisiae |
6 | - |
4°C, 48 h, 21% loss of activity | Saccharomyces cerevisiae |
7 | - |
4°C, 48 h, 5% loss of activity | Saccharomyces cerevisiae |
7.5 | 8 | 4°C, 48 h, stable | Saccharomyces cerevisiae |
9 | - |
4°C, 48 h, 11% loss of activity | Saccharomyces cerevisiae |
10 | - |
4°C, 48 h, 95% loss of activity | Saccharomyces cerevisiae |