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Literature summary for 3.6.1.11 extracted from
- Polyphosphate phosphohydrolase from Endomyces magnusii (1973), Biochim. Biophys. Acta, 321, 336-347.
General Stability
| General Stability | Organism |
|---|---|
| loss of activity after dialysis | Magnusiomyces magnusii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Magnusiomyces magnusii |  |
| Al3+ | - |
Magnusiomyces magnusii | |
| Ca2+ | - |
Magnusiomyces magnusii | |
| Cd2+ | - |
Magnusiomyces magnusii | |
| Cu2+ | - |
Magnusiomyces magnusii | |
| EDTA | - |
Magnusiomyces magnusii | |
| Hg2+ | - |
Magnusiomyces magnusii | |
| KCN | - |
Magnusiomyces magnusii | |
| NaF | - |
Magnusiomyces magnusii | |
| Pb2+ | - |
Magnusiomyces magnusii | |
| PCMB | - |
Magnusiomyces magnusii | |
| phosphate | - |
Magnusiomyces magnusii | |
| UO22+ | - |
Magnusiomyces magnusii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Magnusiomyces magnusii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
| Fe2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
| Mg2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
| Mn2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
| Ni2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
| Zn2+ | Mn2+ or Co2+ required. Mg2+, Zn2+, Fe2+, and Ni2+ are less effective | Magnusiomyces magnusii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Magnusiomyces magnusii | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for months | Magnusiomyces magnusii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (polyphosphate)n + H2O | n = 40, 72, 180 or 290, highest reaction rate, when n is 40 | Magnusiomyces magnusii | (polyphosphate)n-1 + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Magnusiomyces magnusii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 48 | 30°C: about about 60% of maximal activity, 48°C: about 40% of maximal activity | Magnusiomyces magnusii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
15 min, 70% loss of activity | Magnusiomyces magnusii |
| 60 | - |
2 min, complete loss of activity, slight protection from heat inactivation, 10%, by 1 mM MnCl2 | Magnusiomyces magnusii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Magnusiomyces magnusii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.4 | pH 6.2: about 35% of maximal activity, pH 8.4: about 60% of maximal activity | Magnusiomyces magnusii |
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Release 2023.1 (January 2023)
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