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Literature summary for 3.6.1.1 extracted from

  • Belogurov, G.; Malinen, A.; Turkina, M.; Jalonen, U.; Rytkönen, K.; Baykov, A.; Lahti, R.
    Membrane-bound pyrophosphatase of Thermotoga maritima requires sodium for activity (2005), Biochemistry, 44, 2088-2096 .
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D190A the Na+ dose dependency of the D190A variant closely resembles that of the wild-type enzyme Thermotoga maritima
D703N the variant exhibits a much lower slope on the rate vs [Na+] plot than the wild-type enzyme at all of the K+ concentrations examined Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Thermotoga maritima 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the enzyme absolutely requires Na+ for activity, whereas both Na+ and K+ are needed for maximal activity Thermotoga maritima
Li+ Li+ can substitute for Na+ as activator. In the presence of K+, Li+ iss a less potent activator than Na+ Thermotoga maritima
Na+ the enzyme absolutely requires Na+ for activity, whereas both Na+ and K+ are needed for maximal activity Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9S5X0
-
-
Thermotoga maritima DSM 3109 Q9S5X0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Thermotoga maritima 2 phosphate
-
 ?
diphosphate + H2O
-
 Thermotoga maritima DSM 3109 2 phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Tm-PPase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Thermotoga maritima