Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.1 extracted from

  • Hughes, R.C.; Coates, L.; Blakeley, M.P.; Tomanicek, S.J.; Langan, P.; Kovalevsky, A.Y.; Garcia-Ruiz, J.M.; Ng, J.D.
    Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction (2012), Acta Crystallogr. Sect. F, 68, 1482-1487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 cells Thermococcus thioreducens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 25% (w/v) 2-methyl-2,4-pentanediol, 100 mM sodium acetate pH 4.6, 10 mM calcium chloride, 2 mM ammonium sulfate Thermococcus thioreducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20850
-
 x * 20850, calculated from amino acid sequence Thermococcus thioreducens
21000
-
 x * 21000, SDS-PAGE Thermococcus thioreducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Thermococcus thioreducens
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus thioreducens H2L2L6
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and Sephadex S-200 gel filtration Thermococcus thioreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Thermococcus thioreducens 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, SDS-PAGE Thermococcus thioreducens
? x * 20850, calculated from amino acid sequence Thermococcus thioreducens

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
 Thermococcus thioreducens
IPPase
-
 Thermococcus thioreducens