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Literature summary for 3.6.1.1 extracted from

  • Lu, D.; Xie, G.; Gao, R.
    Cloning, purification, and characterization of inorganic pyrophosphatase from the hyperthermophilic archaea Pyrococcus horikoshii (2014), Protein Expr. Purif., 99, 94-98.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0143
-
 diphosphate pH 8.0, 90°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity in the presence of Mg2+, influence of other metal cations is negligible Pyrococcus horikoshii

Organic Solvent Stability

Organic Solvent Comment Organism
urea the enzyme retains 40.66% of the original activity in the presence of 8 M urea after incubation for 24 h at 50°C and 39.3% of the original activity in the presence of 6 M urea at 50°C after incubation for 24 h. It retains 25.96% of the original activity in the presence of 8 M urea after incubation at 50°C for 120 h Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59570
-
-
Pyrococcus horikoshii DSM 12428 O59570
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.6
-
 pH 8.0, 90°C Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates Pyrococcus horikoshii 2 phosphate
-
 ?
diphosphate + H2O the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates Pyrococcus horikoshii DSM 12428 2 phosphate
-
 ?
tetrapolyphosphate + H2O 25.2% activity compared to the activity with diphosphate Pyrococcus horikoshii ?
-
 ?
tetrapolyphosphate + H2O 25.2% activity compared to the activity with diphosphate Pyrococcus horikoshii DSM 12428 ?
-
 ?
tripolyphosphate + H2O 40.3% activity compared to the activity with diphosphate Pyrococcus horikoshii ?
-
 ?
tripolyphosphate + H2O 40.3% activity compared to the activity with diphosphate Pyrococcus horikoshii DSM 12428 ?
-
 ?

Synonyms

Synonyms Comment Organism
PH1907
-
 Pyrococcus horikoshii
PPIase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
88
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 50% of maximal activity, 100°C: about 50% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 the enzyme remains active for 7 days Pyrococcus horikoshii
95
-
 half-life: 5 h Pyrococcus horikoshii
100
-
 half-life: 4 h, the enzyme retains 19.61% of the original activity after incubation at 100°C for 12 h Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3436
-
 diphosphate pH 8.0, 90°C Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.3
-
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11.5 pH 8.5: about 50% of maximal activity, pH 11.5: about 55% of maximal activity Pyrococcus horikoshii