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Literature summary for 3.6.1.1 extracted from
- Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii, a thermoacidophilic archaeon (2011), Extremophiles, 15, 359-364.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes: P103A, P122A, S123A, H125A, S158A, R159A, G163A, H165A, P167A, and D169A in Escherichia coli. The expression level varies from 49 to 165% of that of the wild type enzyme | Sulfurisphaera tokodaii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D169A | mutant enzyme shows 1.8% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| G163A | mutant enzyme shows 3.1% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| H125A | mutant enzyme shows 7.5% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| H165A | mutant enzyme shows 0.4% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| P103A | mutant enzyme shows 3.3% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| P122A | mutant enzyme shows 8.0% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| P167A | mutant enzyme shows 1.8% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| R159A | mutant enzyme shows 1.1% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| S123A | mutant enzyme shows 16% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
| S158A | mutant enzyme shows 2.0% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound enzyme | Sulfurisphaera tokodaii | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sulfurisphaera tokodaii | the enzyme is possibly involved in glycoprotein biosynthesis | ? | - |
? |  |
| additional information | Sulfurisphaera tokodaii 7 | the enzyme is possibly involved in glycoprotein biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | F9VPB8 | - |
- |
| Sulfurisphaera tokodaii 7 | F9VPB8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Sulfurisphaera tokodaii | 2 phosphate | - |
? | |
| diphosphate + H2O | - |
Sulfurisphaera tokodaii 7 | 2 phosphate | - |
? | |
| additional information | the enzyme is possibly involved in glycoprotein biosynthesis | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | the enzyme is possibly involved in glycoprotein biosynthesis | Sulfurisphaera tokodaii 7 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ST2226 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
assay at | Sulfurisphaera tokodaii |
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Release 2023.1 (January 2023)
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