Any feedback?
Literature summary for 3.6.1.1 extracted from
- Isoleucine 259 and isoleucine 260 residues in Streptococcus gordonii soluble inorganic pyrophosphatase play an important role in enzyme activity (2011), J. Biosci. Bioeng., 112, 8-13.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptococcus gordonii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I259D | site-directed mutagenesis, almost inactive mutant | Streptococcus gordonii |
| I259E | site-directed mutagenesis, almost inactive mutant | Streptococcus gordonii |
| I259V | site-directed mutagenesis, the mutant shows 20% reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
| I260D | site-directed mutagenesis, the mutant shows 86% reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
| I260E | site-directed mutagenesis, the mutant shows 89% reduced activity compared to the wild-type enzyme | Streptococcus gordonii |
| I260V | site-directed mutagenesis, the mutant shows 20% increased activity compared to the wild-type enzyme, the mutant is activable by NaF in contrast to the wild-type enzyme | Streptococcus gordonii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.041 | - |
diphosphate | recombinant mutant I260D, pH 9.0, 25°C | Streptococcus gordonii |  |
| 0.054 | - |
diphosphate | recombinant mutants I260V and I260E, pH 9.0, 25°C | Streptococcus gordonii | |
| 0.055 | - |
diphosphate | recombinant mutant I259V, pH 9.0, 25°C | Streptococcus gordonii | |
| 0.062 | - |
diphosphate | recombinant wild-type enzyme, pH 9.0, 25°C | Streptococcus gordonii | |
| 0.163 | - |
diphosphate | recombinant mutant I259D, pH 9.0, 25°C | Streptococcus gordonii | |
| 0.248 | - |
diphosphate | recombinant mutant I259E, pH 9.0, 25°C | Streptococcus gordonii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Streptococcus gordonii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, activates at 2 mM | Streptococcus gordonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+ | Streptococcus gordonii |
| 32000 | - |
1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+ | Streptococcus gordonii |
| 50000 | - |
recombinant mutant I259D, native PAGE, in absence of Mn2+ | Streptococcus gordonii |
| 57000 | - |
1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+ | Streptococcus gordonii |
| 60000 | - |
recombinant mutant I260D, native PAGE, in presence or absence of Mn2+ | Streptococcus gordonii |
| 60000 | - |
recombinant mutants I260E and I259E, native PAGE, in absence of Mn2+ | Streptococcus gordonii |
| 61000 | - |
recombinant mutant I260V, native PAGE, in absence of Mn2+ | Streptococcus gordonii |
| 62000 | - |
recombinant mutant I260E, native PAGE, in presence of Mn2+ | Streptococcus gordonii |
| 64000 | - |
recombinant mutant I260V, native PAGE, in presence of Mn2+ | Streptococcus gordonii |
| 78000 | - |
recombinant wild-type enzyme, native PAGE, in presence of Mn2+ | Streptococcus gordonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus gordonii | P95765 | - |
- |
| Streptococcus gordonii DL1 | P95765 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography | Streptococcus gordonii |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 x * 27000 + 1 * 57000, recombinant mutant I259V, native PAGE in absence of Mn2+, 1 * 32000 + 1 x 62000, recombinant wild-type enzyme, native PAGE in absence of Mn2+ | Streptococcus gordonii |
| More | the hinge region plays an important role in opening and closing of the active site between the N- and C-terminal domains of PPase. Family II PPases are dimer under physiological conditions with Mn2+ present, and may dissociate into monomers at low enzyme concentration in the absence of Mn2+ | Streptococcus gordonii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Streptococcus gordonii |
| PPase | - |
Streptococcus gordonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptococcus gordonii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
diphosphate | recombinant mutant I259D, pH 9.0, 25°C | Streptococcus gordonii | |
| 48 | - |
diphosphate | recombinant mutant I259E, pH 9.0, 25°C | Streptococcus gordonii | |
| 560 | - |
diphosphate | recombinant mutant I260D, pH 9.0, 25°C | Streptococcus gordonii | |
| 570 | - |
diphosphate | recombinant mutant I260E, pH 9.0, 25°C | Streptococcus gordonii | |
| 4420 | - |
diphosphate | recombinant mutant I259V, pH 9.0, 25°C | Streptococcus gordonii | |
| 6290 | - |
diphosphate | recombinant wild-type enzyme, pH 9.0, 25°C | Streptococcus gordonii | |
| 6480 | - |
diphosphate | recombinant mutant I260V, pH 9.0, 25°C | Streptococcus gordonii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Streptococcus gordonii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | family II soluble inorganic pyrophosphatase | Streptococcus gordonii |
| additional information | the hinge region plays an important role in opening and closing of the active site between the N- and C-terminal domains of PPase | Streptococcus gordonii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67.5 | - |
diphosphate | recombinant mutant I259D, pH 9.0, 25°C | Streptococcus gordonii | |
| 193.5 | - |
diphosphate | recombinant mutant I259E, pH 9.0, 25°C | Streptococcus gordonii | |
| 10560 | - |
diphosphate | recombinant mutant I260E, pH 9.0, 25°C | Streptococcus gordonii | |
| 13660 | - |
diphosphate | recombinant mutant I260D, pH 9.0, 25°C | Streptococcus gordonii | |
| 80360 | - |
diphosphate | recombinant mutant I259V, pH 9.0, 25°C | Streptococcus gordonii | |
| 101500 | - |
diphosphate | recombinant wild-type enzyme, pH 9.0, 25°C | Streptococcus gordonii | |
| 120000 | - |
diphosphate | recombinant mutant I260V, pH 9.0, 25°C | Streptococcus gordonii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
