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Literature summary for 3.6.1.1 extracted from
- An ecto-enzyme from Sulfolobus acidocaldarius strain 7 which catalyzes hydrolysis of inorganic pyrophosphate, ATP, and ADP: purification and characterization (1993), J. Biochem., 114, 329-333.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | above 10 mM | Sulfolobus acidocaldarius |  |
| ATP | above 10 mM | Sulfolobus acidocaldarius | |
| diphosphate | above 10 mM | Sulfolobus acidocaldarius | |
| F- | 20 mM, 80% inhibition | Sulfolobus acidocaldarius | |
| additional information | addition of EDTA up to 0.5 mM to the assay mixture does not result in any change in the PPase activity | Sulfolobus acidocaldarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.161 | - |
diphosphate | pH 3.0, 55°C | Sulfolobus acidocaldarius | |
| 0.189 | - |
ATP | pH 3.0, 55°C | Sulfolobus acidocaldarius | |
| 0.237 | - |
ATP | pH 3.0, 55°C | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | 20 mM, 155% stimulation | Sulfolobus acidocaldarius | |
| MgCl2 | 20 mM, 163% stimulation | Sulfolobus acidocaldarius | |
| additional information | divalent cations are not essential for the acid PPase activity | Sulfolobus acidocaldarius | |
| NiCl2 | 20 mM, 156% stimulation | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
1 * 26000, SDS-PAGE | Sulfolobus acidocaldarius |
| 35000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | P50308 | - |
- |
| Sulfolobus acidocaldarius 7 | P50308 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.8 | - |
pH 3.0, 55°C | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | hydrolyzed at 36.2% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
| ADP + H2O | hydrolyzed at 36.2% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
| ATP + H2O | hydrolyzed at 19.4% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
| ATP + H2O | hydrolyzed at 19.4% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
| cyclic tripolyphosphate + H2O | hydrolyzed at 32.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
| cyclic tripolyphosphate + H2O | hydrolyzed at 32.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
| diphosphate + H2O | as far as the release of phosphate, is concerned, diphosphate, is the best substrate | Sulfolobus acidocaldarius | 2 phosphate | - |
? | |
| diphosphate + H2O | as far as the release of phosphate, is concerned, diphosphate, is the best substrate | Sulfolobus acidocaldarius 7 | 2 phosphate | - |
? | |
| tripolyphosphate + H2O | hydrolyzed at 44.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius | ? | - |
? | |
| tripolyphosphate + H2O | hydrolyzed at 44.3% of the rate compared to diphosphate | Sulfolobus acidocaldarius 7 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 26000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid PPase | - |
Sulfolobus acidocaldarius |
| D1C enzyme | - |
Sulfolobus acidocaldarius |
| inorganic pyrophosphatase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Sulfolobus acidocaldarius |
| 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the purified enzyme is less thermostable than the membrane-bound enzyme in situ. The remaining activity after incubation at 70°C, pH 7, for 15 min, is about 70 and 100% for the purified enzyme and the membrane enzyme, respectively | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
assay at | Sulfolobus acidocaldarius |
| 3 | 3.5 | - |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 4.5 | at pH 2.5 and 4.5, about 50% of the maximal activity is observed | Sulfolobus acidocaldarius |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Sulfolobus acidocaldarius | addition of phosphate and/or diphosphate, to the semi-synthetic media results in about twofold increase in the acid PPase activity. However, in the nutrient broth, no induction is observed even when diphosphate is supplemented | up |
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