Any feedback?
Literature summary for 3.6.1.1 extracted from
- Identification and characterization of inorganic pyrophosphatase and PAP phosphatase from Thermococcus onnurineus NA1 (2009), J. Bacteriol., 191, 3415-3419.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3)pLysS cells | Thermococcus onnurineus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0188 | - |
diphosphate | pH and temperature not specified in the publication | Thermococcus onnurineus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 15% activity in the presence of 50 mM Co2+ compared to 50 mM Mg2+ | Thermococcus onnurineus | |
| Mg2+ | maximal activity in the presence of 0.2 mM Mg2+ | Thermococcus onnurineus | |
| additional information | not affected by 50 mM Mn2+, Ni2+, Ca2+, Zn2+, and Cu2+ | Thermococcus onnurineus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Thermococcus onnurineus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus onnurineus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity chromatography | Thermococcus onnurineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-AMP + H2O | 4% activity compared to diphosphate | Thermococcus onnurineus | adenosine + phosphate | - |
? | |
| ADP + H2O | 5% activity compared to diphosphate | Thermococcus onnurineus | AMP + phosphate | - |
? | |
| ATP + H2O | 4% activity compared to diphosphate | Thermococcus onnurineus | ADP + phosphate | - |
? | |
| diphosphate + H2O | 100% activity | Thermococcus onnurineus | phosphate + phosphate | - |
? | |
| additional information | no activity with PAP, PAPS, 3'-CMP, fructose 1,6-bisphosphate, and D-myo-inositol 1-monophosphate | Thermococcus onnurineus | ? | - |
? | |
| polyphosphate + H2O | less than 1% activity compared to diphosphate | Thermococcus onnurineus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Thermococcus onnurineus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inorganic pyrophosphatase | - |
Thermococcus onnurineus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
diphosphate | pH and temperature not specified in the publication | Thermococcus onnurineus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Thermococcus onnurineus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
