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Literature summary for 3.6.1.1 extracted from

  • Mu, H.; Zhou, S.M.; Xia, Y.; Zou, H.; Meng, F.; Yan, Y.B.
    Inactivation and unfolding of the hyperthermophilic inorganic pyrophosphatase from Thermus thermophilus by sodium dodecyl sulfate (2009), Int. J. Mol. Sci., 10, 2849-2859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
SDS SDS acts as a mixed-type reversible inhibitor of PPase, PPase can be fully inactivated at SDS concentration of 2 mM Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P38576
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P38576
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Thermus thermophilus phosphate + phosphate
-
?
diphosphate + H2O
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Thermus thermophilus
PPase
-
Thermus thermophilus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11
-
at 25°C, pH not specified in the publication Thermus thermophilus SDS

General Information

General Information Comment Organism
metabolism PPase is an essential constitutive enzyme for energy metabolism and clearance of excess diphosphate Thermus thermophilus