Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | SDS acts as a mixed-type reversible inhibitor of PPase, PPase can be fully inactivated at SDS concentration of 2 mM | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P38576 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | P38576 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 200 gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Thermus thermophilus | phosphate + phosphate | - |
? | |
diphosphate + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Thermus thermophilus |
PPase | - |
Thermus thermophilus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.11 | - |
at 25°C, pH not specified in the publication | Thermus thermophilus | SDS |
General Information | Comment | Organism |
---|---|---|
metabolism | PPase is an essential constitutive enzyme for energy metabolism and clearance of excess diphosphate | Thermus thermophilus |