Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.1 extracted from

  • Hoelzle, K.; Peter, S.; Sidler, M.; Kramer, M.M.; Wittenbrink, M.M.; Felder, K.M.; Hoelzle, L.E.
    Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis (2010), BMC Microbiol., 10, 194.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia LMG194 cells Mycoplasma suis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ enzymatic activity is inhibited more than 95% in the presence of 5 mM Ca2+ Mycoplasma suis
EDTA enzymatic activity is inhibited more than 70% in the presence of 5 mM EDTA Mycoplasma suis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mycoplasma suis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the activity of the enzyme is strongly dependent on Mg2+. High enzyme activity is found between 1 and 100 mM Mg2+ with a maximum activity at 10 mM. At pH 7.5, the maximum PPase activity is at 50 mM Mg2+ Mycoplasma suis
Mn2+ substitution of Mg2+ cations with Mn2+ results in significantly lower activity of 25.34% of the Mg2+-induced activity Mycoplasma suis
Zn2+ substitution of Mg2+ cations with Zn2+ results in significantly lower activity of 14.3% of the Mg2+-induced activity Mycoplasma suis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18600
-
4 * 18600, calculated from amino acid sequence Mycoplasma suis
20000
-
4 * 20000, SDS-PAGE Mycoplasma suis
80000
-
gel filtration Mycoplasma suis

Organism

Organism UniProt Comment Textmining
Mycoplasma suis C4RA40
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mycoplasma suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Mycoplasma suis phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 20000, SDS-PAGE Mycoplasma suis
homotetramer 4 * 18600, calculated from amino acid sequence Mycoplasma suis

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Mycoplasma suis
PPase
-
Mycoplasma suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Mycoplasma suis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 at conditions below pH 7.5 and above pH 10.0 activity decreases considerably Mycoplasma suis

pI Value

Organism Comment pI Value Maximum pI Value
Mycoplasma suis calculated from amino acid sequence
-
4.7