Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the purified enzyme on hydrophobic supports polycephamide and lysopolycephamide allows preservation of 24.2% and 17.3% of the starting catalytic activities with activity of imMobilized enzymes per gram support of 507 and 285 U/g, respectively | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at up to 1 mM, inhibits at higher concentrations, complete inhibition at 2 mM, overview | Gossypium hirsutum | |
Co2+ | activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum | |
fluoride | 50% inhibition at 0.5 mM, 70-80% at 1.0 mM | Gossypium hirsutum | |
Mn2+ | activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum | |
Zn2+ | activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at up to 1 mM, inhibits at higher concentrations, complete inhibition at 2 mM, overview | Gossypium hirsutum | |
Co2+ | activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum | |
Mg2+ | 7-8fold activation | Gossypium hirsutum | |
Mn2+ | activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum | |
Zn2+ | highly activates at up to 1 mM, inhibits at higher concentrations, overview | Gossypium hirsutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Gossypium hirsutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Gossypium hirsutum | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | - |
var. 108-F | - |
Purification (Comment) | Organism |
---|---|
native enzyme from seedlings 30fold by acetone precipitation, gel filtration, and anion exchange chromatography | Gossypium hirsutum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | the activity of inorganic diphosphatase reaches its maximum 1 day after the beginning of seed bud development, and begins to decrease after 2 days with 34% of its maximum value after 3 days of seed bud development | Gossypium hirsutum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.0185 mMol/g of tissue after 2 days of seed bud development | Gossypium hirsutum |
240 | - |
purified native enzyme | Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Gossypium hirsutum | 2 phosphate | - |
? | |
diphosphate + H2O | the enzyme is very specific for diphosphate as substrate, substrate specificity, overview | Gossypium hirsutum | 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Gossypium hirsutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | - |
Gossypium hirsutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, 70-80% remaining activity | Gossypium hirsutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
pH optimum in absence of Mg2+ | Gossypium hirsutum |
8.6 | - |
pH optimum in presence of Mg2+ | Gossypium hirsutum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
additional information | pH 7.2, 37°C, fluoride inhibition kinetics | Gossypium hirsutum |