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Literature summary for 3.6.1.1 extracted from

  • Hansen, T.; Urbanke, C.; Leppänen, V.M.; Goldman, A.; Brandenburg, K.; Schäfer, G.
    The extreme thermostable pyrophosphatase from Sulfolobus acidocaldarius: enzymatic and comparative biophysical characterization (1999), Arch. Biochem. Biophys., 363, 135-147.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius

Crystallization (Commentary)

Crystallization (Comment) Organism
CD and FTIR spectra demonstrate a similar overall fold for enzyme and PPases from Escherichia coli and Thermus thermophilus Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 diphosphate pH 7.0, 75°C Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2% of the activity with Mg2+ Sulfolobus acidocaldarius
Co2+ 88% of the activity with Mg2+ Sulfolobus acidocaldarius
Mg2+ preferred divalent cation, Km value 0.9 mM Sulfolobus acidocaldarius
Mn2+ 32% of the activity with Mg2+ Sulfolobus acidocaldarius
additional information divalent cation required. Activity in decreasing order: Mg2+, Zn2+, Co2+, Mn2+, Ni2+, Ca2+ Sulfolobus acidocaldarius
Ni2+ 3% of the activity with Mg2+ Sulfolobus acidocaldarius
Zn2+ 95% of the activity with Mg2+ Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19365
-
 x * 19365, calculated Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
860
-
 pH 7.0, 75°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
ATP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
diphosphate + H2O
-
 Sulfolobus acidocaldarius 2 phosphate
-
 ?
ITP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
phosphoenolpyruvate + H2O 1-2% of the activity with diphosphate Sulfolobus acidocaldarius pyruvate + phosphate
-
 ?
triphosphate + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
TTP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 19365, calculated Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
89
-
 melting temperature, presence of EDTA Sulfolobus acidocaldarius
95
-
 half-life of catalytic activity 2.5 h Sulfolobus acidocaldarius
99
-
 melting temperature, presence of Mg2+ Sulfolobus acidocaldarius
100
-
 melting temperature above 100°C, prensence of Mn2+ Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1700
-
 diphosphate pH 7.0, 75°C Sulfolobus acidocaldarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9
-
 Sulfolobus acidocaldarius