Any feedback?
Literature summary for 3.6.1.1 extracted from
- A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes (2006), Biochem. J., 395, 211-221.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ppa1, isozyme PPase 1, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli | Arabidopsis thaliana |
| gene ppa1, isozyme sPPase-I, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Chlamydomonas reinhardtii |
| gene ppa2, isozyme sPPase-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Chlamydomonas reinhardtii |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| chloroplast | isozyme sPPase-I | Chlamydomonas reinhardtii | 9507 | - |
| mitochondrion | isozyme sPPase-II | Chlamydomonas reinhardtii | 5739 | - |
| additional information | isozyme subcellular localization study | Chlamydomonas reinhardtii | - |
- |
| soluble | isozyme sPPase-I | Chlamydomonas reinhardtii | - |
- |
| soluble | isozyme sPPase-II | Chlamydomonas reinhardtii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
| Fe2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
| Mg2+ | required, best divalent cation | Chlamydomonas reinhardtii | |
| Mn2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
| Zn2+ | can partially, upt to 30%, substitute for Mg2+ | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
| 24350 | - |
1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
| 28000 | 32000 | gel filtration and native PAGE | Chlamydomonas reinhardtii |
| 29850 | - |
1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
| 30000 | - |
1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
| 33000 | 37000 | gel filtration and native PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Chlamydomonas reinhardtii | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LXC9 | isozyme PPA1, chloroplast precursor; isozyme PPA1 | - |
| Chlamydomonas reinhardtii | Q93Y52 | ppa1, chloroplast precursor; soluble inorganic pyrophosphatase isozyme I, plastidial isozyme sPPase-I, gene ppa1, is the major isozyme | - |
| Chlamydomonas reinhardtii | Q949J1 | ppa2; soluble inorganic pyrophosphatase isozyme II, minor isozyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the chloroplast isozyme I contains a precursor sequence, the recombinant enzyme is processed in bacterial cells | Chlamydomonas reinhardtii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozyme sPPase-I by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration | Chlamydomonas reinhardtii |
| native isozyme sPPase-II by streptomycin and ammonium sulfate fractionation, dialysis, anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography and gel filtration | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Chlamydomonas reinhardtii | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, isozyme sPPase-II, SDS-PAGE, 1 * 24350, isozyme sPPase-II, mass spectrometry | Chlamydomonas reinhardtii |
| monomer | 1 * 30000, isozyme sPPase-I, SDS-PAGE, 1 * 29850, isozyme sPPase-I, mass spectrometry | Chlamydomonas reinhardtii |
| More | peptide mass fingerprint analysis of isozyme sPPase-I | Chlamydomonas reinhardtii |
| More | peptide mass fingerprint analysis of isozyme sPPase-II | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chloroplast inorganic pyrophosphatase 1 | - |
Arabidopsis thaliana |
| More | the enzyme is a member of the sPPase family I | Chlamydomonas reinhardtii |
| PPA1 | - |
Arabidopsis thaliana |
| PPase 1 | - |
Chlamydomonas reinhardtii |
| PPase 1 | - |
Arabidopsis thaliana |
| PPase 2 | - |
Chlamydomonas reinhardtii |
| pyrophosphate phospho-hydrolase 1 | - |
Arabidopsis thaliana |
| soluble inorganic pyrophosphatase | - |
Chlamydomonas reinhardtii |
| soluble inorganic pyrophosphatase 2 | - |
Chlamydomonas reinhardtii |
| sPPAse | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chlamydomonas reinhardtii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
