Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pyrococcus horikoshii | |
Co2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii | |
fluoride | potent inhibition, IC50: 0.1 mM | Pyrococcus horikoshii | |
Mn2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii | |
Zn2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
diphosphate | pH 7.5, 60°C, recombinat enzyme | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii | |
Mg2+ | required, best divalent cation, 5 mM, Km is 0.303 mM | Pyrococcus horikoshii | |
Mn2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii | |
additional information | no activation by Ni2+ | Pyrococcus horikoshii | |
Zn2+ | can substitute for Mg2+ at concentrations up to 0.5 mM, inhibitory above | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20833 | - |
x * 24500, recombinant enzyme, SDS-PAGE, x * 20833, amino acid sequence calculation | Pyrococcus horikoshii |
24500 | - |
x * 24500, recombinant enzyme, SDS-PAGE, x * 20833, amino acid sequence calculation | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Pyrococcus horikoshii | - |
2 phosphate | - |
? | |
diphosphate + H2O | Pyrococcus horikoshii OT-3 | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59570 | strain OT3 | - |
Pyrococcus horikoshii OT-3 | O59570 | strain OT3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Pyrococcus horikoshii | 2 phosphate | - |
? | |
diphosphate + H2O | specific for | Pyrococcus horikoshii | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Pyrococcus horikoshii OT-3 | 2 phosphate | - |
? | |
diphosphate + H2O | specific for | Pyrococcus horikoshii OT-3 | 2 phosphate | - |
? | |
additional information | triphosphate, ATP, and ADP are poor substrates, AMP, phosphoenolpyruvate, and 4-nitrophenyl phosphate are no substrates | Pyrococcus horikoshii | ? | - |
? | |
additional information | triphosphate, ATP, and ADP are poor substrates, AMP, phosphoenolpyruvate, and 4-nitrophenyl phosphate are no substrates | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24500, recombinant enzyme, SDS-PAGE, x * 20833, amino acid sequence calculation | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
family I inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
family I PPase | - |
Pyrococcus horikoshii |
PPase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | activity range, high activity at 60-75°C | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
half-life about 50 min, recombinant enzyme | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
744 | - |
diphosphate | pH 7.5, 60°C, recombinat enzyme | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus horikoshii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
potent inhibition, IC50: 0.1 mM | Pyrococcus horikoshii | fluoride |