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Literature summary for 3.6.1.1 extracted from

  • Zyryanov, A.B.; Shestakov, A.S.; Lahti, R.; Baykov, A.A.
    Mechanism by which metal cofactors control substrate specificity in pyrophosphatase (2002), Biochem. J., 367, 901-906.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.1

Protein Variants

Protein Variants Comment Organism
K193R decrease in activity Saccharomyces cerevisiae
K56R large decrease in activity Saccharomyces cerevisiae
R78K increased activity Saccharomyces cerevisiae
Y93F large decrease in activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP competitive Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae
Mg2+ required Escherichia coli
Mg2+ required Rattus norvegicus
Mg2+ required Saccharomyces cerevisiae
Mg2+ required Streptococcus mutans
Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Escherichia coli
Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Rattus norvegicus
Mn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae
Zn2+ supports the hydrolysis of ATP and tripolyphosphate, very weak activation of diphosphate hydrolysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
diphosphate + H2O Rattus norvegicus important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
diphosphate + H2O Saccharomyces cerevisiae important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
diphosphate + H2O Streptococcus mutans important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?
diphosphate + H2O Escherichia coli MRE 600 important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MRE 600
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Streptococcus mutans
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O reaction only in the presence of Mn2+ Escherichia coli ?
-
 ?
ATP + H2O reaction only in the presence of Mn2+ Rattus norvegicus ?
-
 ?
ATP + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ?
-
 ?
ATP + H2O reaction only in the presence of Mn2+ Escherichia coli MRE 600 ?
-
 ?
diphosphate + H2O
-
 Escherichia coli 2 phosphate
-
 ?
diphosphate + H2O
-
 Rattus norvegicus 2 phosphate
-
 ?
diphosphate + H2O
-
 Saccharomyces cerevisiae 2 phosphate
-
 ?
diphosphate + H2O
-
 Streptococcus mutans 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Rattus norvegicus 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Saccharomyces cerevisiae 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Streptococcus mutans 2 phosphate
-
 ?
diphosphate + H2O
-
 Escherichia coli MRE 600 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli MRE 600 2 phosphate
-
 ?
additional information no hydrolysis of ATP and traces of activity with tripolyphosphate in the presence of Mg2+ Saccharomyces cerevisiae ?
-
 ?
additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli ?
-
 ?
additional information no hydrolysis of ATP in the presence of Mg2+ Rattus norvegicus ?
-
 ?
additional information no hydrolysis of ATP in the presence of Mg2+ Streptococcus mutans ?
-
 ?
additional information no hydrolysis of ATP in the presence of Mg2+ Escherichia coli MRE 600 ?
-
 ?
tripolyphosphate + H2O reaction only in the presence of Mn2+ or Zn2+ Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 ATP less than 0.005/sec, pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae
0.05
-
 tripolyphosphate pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae
260
-
 diphosphate pH 7.2, 25°C, in the presence of Mg2+ Saccharomyces cerevisiae