Protein Variants | Comment | Organism |
---|---|---|
D97E | decreased pH-independence rates of diphosphate hydrolysis and resynthesis, destabilized EMg2(Mgdiphosphate)2 complex, raised pKa, Mg2+ binding changed | Escherichia coli |
E20D | lower specific activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
diphosphate | pH 7.2, E20D | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | wild type: two Mg2+ ions per active site required for catalysis | Escherichia coli | |
Mg2+ | stabilization of the hexameric E20D enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | wild type | Escherichia coli |
trimer | dissociated variant, SDS-PAGE | Escherichia coli |