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Literature summary for 3.6.1.1 extracted from
- Methanococcus jannaschii ORF mj0608 codes for a class C inorganic pyrophosphatase protected by Co2+ or Mn2+ ions against fluoride inhibition (2000), Arch. Biochem. Biophys., 379, 292-298.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Methanocaldococcus jannaschii |  |
| Cu2+ | - |
Methanocaldococcus jannaschii | |
| F- | Co2+ and Mn2+ protect against fluoride inhibition | Methanocaldococcus jannaschii | |
| Fe2+ | - |
Methanocaldococcus jannaschii | |
| Zn2+ | inhibition caused at a separate site rather than as Zn-diphosphate substrate | Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | protects against fluoride inhibition | Methanocaldococcus jannaschii | |
| Mg2+ | - |
Methanocaldococcus jannaschii | |
| Mn2+ | protects against fluoride inhibition | Methanocaldococcus jannaschii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Methanocaldococcus jannaschii | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 600 | - |
at 25°C | Methanocaldococcus jannaschii |
| 8000 | - |
at 85°C | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Methanocaldococcus jannaschii | 2 phosphate | - |
? | |
| diphosphate + H2O | high specificity | Methanocaldococcus jannaschii | 2 phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
growth optimum for organism and highest activity | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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