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Literature summary for 3.5.99.9 extracted from

  • Qu, Y.; Spain, J.C.
    Catabolic pathway for 2-nitroimidazole involves a novel nitrohydrolase that also confers drug resistance (2011), Environ. Microbiol., 13, 1010-1017.
    View publication on PubMed
Search for more literature for 3.5.99.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mycobacterium sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 1 mM, stimulation Mycobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-nitroimidazole + H2O Mycobacterium sp.
-
 imidazol-2-one + nitrite
-
 ?
2-nitroimidazole + H2O Mycobacterium sp. JS330
-
 imidazol-2-one + nitrite
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium sp. F4ZCI3
-
-
Mycobacterium sp. JS330 F4ZCI3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
 substrate benznidazole, pH 7.2, 24°C Mycobacterium sp.
0.0236
-
 substrate 2-nitroimidazole, pH 7.2, 24°C Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitroimidazole + H2O
-
 Mycobacterium sp. imidazol-2-one + nitrite
-
 ?
2-nitroimidazole + H2O
-
 Mycobacterium sp. JS330 imidazol-2-one + nitrite
-
 ?
benznidazole + H2O
-
 Mycobacterium sp. N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
 ?
benznidazole + H2O
-
 Mycobacterium sp. JS330 N-benzyl-2-(2-oxo-2,3-dihydro-1H-imidazol-1-yl)acetamide + nitrite
-
 ?
additional information no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide Mycobacterium sp. ?
-
 ?
additional information no activity with 4-nitroimidazole, metronidazole, 2,4-dinitroimidazole, 3-nitro-1,2,4-triazol-5-one, nitazoxanide, or tizoxanide Mycobacterium sp. JS330 ?
-
 ?

Synonyms

Synonyms Comment Organism
2NI denitrase
-
 Mycobacterium sp.
2NI nitrohydrolase
-
 Mycobacterium sp.
NnhA
-
 Mycobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
-
 Mycobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Mycobacterium sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors required, no oxygen consumption Mycobacterium sp.

General Information

General Information Comment Organism
metabolism Initial step in the biodegradation of 2-nitroimidazole by Mycobacterium sp. JS330. This is the first hydrolytic enzyme involved in elimination of the nitro group. The organism grows on 2-nitroimidazole as sole carbon source. Mycobacterium sp.