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Literature summary for 3.5.99.6 extracted from

  • Naderer, T.; Heng, J.; McConville, M.
    Evidence that intracellular stages of Leishmania major utilize amino sugars as a major carbon source (2010), PLoS Pathog., 6, e1001245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.99.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of native GND, but not a cytosolic form of GND, in DELTAgnd parasites restores hexosamine-dependent growth. Targeted expression of glucosamine-6-phosphate deaminase, GND, in glycosomes via a canonical C-terminal targeting signal when expressed as a GFP fusion protein Leishmania major

Protein Variants

Protein Variants Comment Organism
additional information generation of enzyme-deficient DELTAgnd mutants, phenotype, detailed overview Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Leishmania major
-
promastigote
-
 Leishmania major
-

Synonyms

Synonyms Comment Organism
gnd
-
 Leishmania major

General Information

General Information Comment Organism
malfunction mutant parasites lacking GND are unable to grow in medium containing amino sugars as sole carbohydrate source and rapidly loose viability, concomitant with the hyper-accumulation of hexosamine-phosphates. Expression of native GND, but not a cytosolic form of GND, in DELTAgnd parasites restored hexosamine-dependent growth, indicating that toxicity is due to depletion of glycosomal pools of ATP. Promastigote and amastigote stages of the DELTAgnd mutant are unable to replicate within macrophages and were either completely cleared or exhibited reduced lesion development in highly susceptible Balb/c mice Leishmania major