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Literature summary for 3.5.99.6 extracted from

  • Sosa-Peinado, A.; Gonzalez-Andrade, M.
    Site-directed fluorescence labeling reveals differences on the R-conformer of glucosamine 6-phosphate deaminase of Escherichia coli induced by active or allosteric site ligands at steady state (2005), Biochemistry, 44, 15083-15092.
    View publication on PubMed
Search for more literature for 3.5.99.6

Protein Variants

Protein Variants Comment Organism
C118S/C228S/C239S decrease in kcat-value, no modification of allosteric activation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-2-amino-D-glucitol 6-phosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 D-glucosamine 6-phosphate mutant C118S/C228S/C239S, pH 7.5, 30°C Escherichia coli
0.49
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C Escherichia coli
0.5
-
 D-glucosamine 6-phosphate wild-type, pH 7.5, 30°C Escherichia coli
0.51
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C Escherichia coli
0.52
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C164, pH 7.5, 30°C Escherichia coli
0.52
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C206, pH 7.5, 30°C Escherichia coli
4.6
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C206, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli
5.1
-
 D-glucosamine 6-phosphate mutant C118S/C228S/C239S, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli
5.1
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C164, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli
5.1
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli
5.2
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli
5.2
-
 D-glucosamine 6-phosphate wild-type, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A759
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-fructose 6-phosphate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C164, pH 7.5, 30°C Escherichia coli
69.5
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C206, pH 7.5, 30°C Escherichia coli
70.6
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C Escherichia coli
70.9
-
 D-glucosamine 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C Escherichia coli
71.6
-
 D-glucosamine 6-phosphate wild-type, modified with bimane at C164, pH 7.5, 30°C Escherichia coli
96
-
 D-glucosamine 6-phosphate mutant C118S/C228S/C239S, pH 7.5, 30°C Escherichia coli
153
-
 D-glucosamine 6-phosphate wild-type, pH 7.5, 30°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00223
-
 2-deoxy-2-amino-D-glucitol 6-phosphate wild-type, pH 7.5, 30°C Escherichia coli
0.0035
-
 2-deoxy-2-amino-D-glucitol 6-phosphate wild-type, modified with bimane at C206, pH 7.5, 30°C Escherichia coli
0.0036
-
 2-deoxy-2-amino-D-glucitol 6-phosphate wild-type, modified with bimane at C164, pH 7.5, 30°C Escherichia coli
0.0038
-
 2-deoxy-2-amino-D-glucitol 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C Escherichia coli
0.0041
-
 2-deoxy-2-amino-D-glucitol 6-phosphate wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C Escherichia coli
0.0043
-
 2-deoxy-2-amino-D-glucitol 6-phosphate mutant C118S/C228S/C239S, pH 7.5, 30°C Escherichia coli