Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.99.6 extracted from

  • Montero-Moran, G.M.; Horjales, E.; Calcagno, M.L.; Altamirano, M.M.
    Tyr254 hydroxyl group acts as a two-way switch mechanism in the coupling of heterotrophic and homotropic effects in Escherichia coli glucosamine-6-phosphate deaminase (1998), Biochemistry, 37, 7844-7849.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-acetylglucosamine 6-phosphate
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y254F the mutant is less active than wild-type enzyme, the replacement causes an uncoupling of the homotropic and heterotrophic effects Escherichia coli
Y254T the mutation results in pure K-system with a similar catalytic activity to that of the wild-type enzyme, mutant displays kcat values similar to the wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and two mutants Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine 6-phosphate + H2O
-
Escherichia coli D-fructose 6-phosphate + NH3
-
?

Subunits

Subunits Comment Organism
hexamer enzyme is a dimer of trimers Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.4
-
D-glucosamine 6-phosphate pH 7.7, 30°C, mutant enzyme Y254F Escherichia coli
75
-
D-glucosamine 6-phosphate pH 7.7, 30°C, mutant enzyme Y254F in presence of N-acetylglucosamine 6-phosphate Escherichia coli
218
-
D-glucosamine 6-phosphate pH 7.7, 30°C, mutant enzyme Y254T Escherichia coli
248
-
D-glucosamine 6-phosphate pH 7.7, 30°C, mutant enzyme Y254T in presence of N-acetylglucosamine 6-phosphate Escherichia coli
292
-
D-glucosamine 6-phosphate pH 7.7, 30°C, wild-type enzyme Escherichia coli
320
-
D-glucosamine 6-phosphate pH 7.7, 30°C, wild-type enzyme in presence of N-acetylglucosamine 6-phosphate Escherichia coli