Application | Comment | Organism |
---|---|---|
molecular biology | nbzE, involved in the ring cleavage pathway of 2-aminophenol, is localized on the 6.6 kb SnaBI-SmaI fragment of the plasmid pNB1 and clusters in the order nbzC-nbzD-nbzE as an operon | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
cloned into plasmid pBR328, expression in Escherichia coli JM109 harboring plasmids pHS29, pHS293, pHS295, pHS291, pHS2915 and PHS2916 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | spontaneous mutants HS121(pNB2) during serial transfer on nonselective agar plates, can not grow on nitrobenzene as the sole source of carbon, nitrogen and energy, have lost plasmid pNB1, plasmid-cured HS124 mutant, obtained by incubating the parent strain at an elevated temperature, have lost plasmid pNB2 and also can not grow on nitrobenzene | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida HS12 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate + H2O | part of the nitrobenzene degradation pathway | Pseudomonas putida | 4-oxalocrotonate + NH3 | - |
? | |
2-aminomuconate + H2O | part of the nitrobenzene degradation pathway | Pseudomonas putida HS12 | 4-oxalocrotonate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-AM deaminase | - |
Pseudomonas putida |
2-aminomuconate deaminase | - |
Pseudomonas putida |
nbzE | - |
Pseudomonas putida |