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Literature summary for 3.5.99.5 extracted from

  • Park, H.S.; Kim, H.S.
    Identification and characterization of the nitrobenzene catabolic plasmids pNB1 and pNB2 in Pseudomonas putida HS12 (2000), J. Bacteriol., 182, 573-580.
    View publication on PubMed
Search for more literature for 3.5.99.5

Application

Application Comment Organism
molecular biology nbzE, involved in the ring cleavage pathway of 2-aminophenol, is localized on the 6.6 kb SnaBI-SmaI fragment of the plasmid pNB1 and clusters in the order nbzC-nbzD-nbzE as an operon Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
cloned into plasmid pBR328, expression in Escherichia coli JM109 harboring plasmids pHS29, pHS293, pHS295, pHS291, pHS2915 and PHS2916 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information spontaneous mutants HS121(pNB2) during serial transfer on nonselective agar plates, can not grow on nitrobenzene as the sole source of carbon, nitrogen and energy, have lost plasmid pNB1, plasmid-cured HS124 mutant, obtained by incubating the parent strain at an elevated temperature, have lost plasmid pNB2 and also can not grow on nitrobenzene Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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-
-
Pseudomonas putida HS12
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate + H2O part of the nitrobenzene degradation pathway Pseudomonas putida 4-oxalocrotonate + NH3
-
 ?
2-aminomuconate + H2O part of the nitrobenzene degradation pathway Pseudomonas putida HS12 4-oxalocrotonate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
2-AM deaminase
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 Pseudomonas putida
2-aminomuconate deaminase
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 Pseudomonas putida
nbzE
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 Pseudomonas putida