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Literature summary for 3.5.99.5 extracted from

  • He, Z.; Spain, J.C.
    A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45 (1998), J. Bacteriol., 180, 2502-2506.
    View publication on PubMed
Search for more literature for 3.5.99.5

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 2 mM, 20% inhibition Ectopseudomonas oleovorans
diethyldicarbonate 2 mM, complete inhibition Ectopseudomonas oleovorans
MnCl2 2 mM, 20% inhibition Ectopseudomonas oleovorans
additional information no inhibition by 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Ectopseudomonas oleovorans
phenylhydrazine 10 mM, 20% inhibition Ectopseudomonas oleovorans
ZnCl2 2 mM, 70% inhibition Ectopseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 2-aminomuconate
-
 Ectopseudomonas oleovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Ectopseudomonas oleovorans 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Ectopseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16600
-
 6 * 16600, SDS-PAGE Ectopseudomonas oleovorans
100000
-
 gel filtration Ectopseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminomuconate + H2O Ectopseudomonas oleovorans intermediate in biodegradation of nitrobenzene ?
-
 ?
2-aminomuconate + H2O Ectopseudomonas oleovorans JS45 intermediate in biodegradation of nitrobenzene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ectopseudomonas oleovorans
-
 JS45
-
Ectopseudomonas oleovorans JS45
-
 JS45
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ectopseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.8
-
-
 Ectopseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate + H2O
-
 Ectopseudomonas oleovorans 4-oxalocrotonate + NH3
-
 ir
2-aminomuconate + H2O
-
 Ectopseudomonas oleovorans JS45 4-oxalocrotonate + NH3
-
 ir
2-aminomuconate + H2O intermediate in biodegradation of nitrobenzene Ectopseudomonas oleovorans ?
-
 ?
2-aminomuconate + H2O intermediate in biodegradation of nitrobenzene Ectopseudomonas oleovorans JS45 ?
-
 ?
additional information no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Ectopseudomonas oleovorans ?
-
 ?
additional information no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Ectopseudomonas oleovorans JS45 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 16600, SDS-PAGE Ectopseudomonas oleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 stable for at least 3 days Ectopseudomonas oleovorans
60
-
 77% loss of activity in 5 min Ectopseudomonas oleovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
208
-
 2-aminomuconate
-
 Ectopseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Ectopseudomonas oleovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 8.8 at room temperature for at least 3 h Ectopseudomonas oleovorans