Literature summary for 3.5.99.2 extracted from

Begum, A.; Drebes, J.; Kikhney, A.; Mueller, I.B.; Perbandt, M.; Svergun, D.; Wrenger, C.; Betzel, C.
Staphylococcus aureus thiaminase II: oligomerization warrants proteolytic protection against serine proteases (2013), Acta Crystallogr. Sect. D, 69, 2320-2329.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BLR (DE3) cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
D111A/K115A	the activity of the monomeric mutant is not significantly altered in comparison to the wild type enzyme	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.4	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
2.25	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	4 * 24000, SDS-PAGE	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-amino-5-aminomethyl-2-methylpyrimidine + H2O	Staphylococcus aureus	-	4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3	-	?
thiamine + H2O	Staphylococcus aureus	-	4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Strep-Tactin Sepharose column chromatography and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-amino-5-aminomethyl-2-methylpyrimidine + H2O	-	Staphylococcus aureus	4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3	-	?
thiamine + H2O	-	Staphylococcus aureus	4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 24000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
TenA	-	Staphylococcus aureus
thiaminase II	-	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00407	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
0.0068	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00292	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
0.00304	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

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Release 2023.1 (January 2023)