Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.99.2 extracted from

  • Ikehata, H.
    The purification of thiaminase II (1960), J. Gen. Appl. Microbiol., 6, 30-39.
No PubMed abstract available
Search for more literature for 3.5.99.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus
L-cysteine in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus

Crystallization (Commentary)

Crystallization (Comment) Organism
dialyzing the purified enzyme solution against 52% saturated ammonium sulfate solution, two weeks Aneurinibacillus aneurinilyticus

Inhibitors

Inhibitors Comment Organism Structure
As2O3 0.01 mM 33% inhibition Aneurinibacillus aneurinilyticus
Ca2+ 0.01 mM 45% inhibition Aneurinibacillus aneurinilyticus
Cd2+ 0.01 mM 88% inhibition Aneurinibacillus aneurinilyticus
Cu2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus
Fe2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus
Fe3+ 0.01 mM 45% inhibition Aneurinibacillus aneurinilyticus
ICH2COOH 0.01 mM 42% inhibition Aneurinibacillus aneurinilyticus
Li+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
Mg2+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
Mn2+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
Ni2+ 0.01 mM 73% inhibition Aneurinibacillus aneurinilyticus
p-chloromercuribenzoate 0.01 mM 99% inhibition Aneurinibacillus aneurinilyticus
Zn2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 thiamine
-
 Aneurinibacillus aneurinilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiamine + H2O Aneurinibacillus aneurinilyticus
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

Organism UniProt Comment Textmining
Aneurinibacillus aneurinilyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aneurinibacillus aneurinilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine + H2O
-
 Aneurinibacillus aneurinilyticus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Aneurinibacillus aneurinilyticus

pH Range

pH Minimum pH Maximum Comment Organism
6.6 9.4
-
 Aneurinibacillus aneurinilyticus