Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
diethyl dicarbonate | 2 mM, complete loss of activity | Pseudomonas oleovorans | |
Mn2+ | 2 mM, 20% inhibition | Pseudomonas oleovorans | |
additional information | not inhibitory: saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid, or EDTA at 25 mM | Pseudomonas oleovorans | |
phenylhydrazine | 10 mM, 80% inhibition | Pseudomonas oleovorans | |
Zn2+ | 2 mM, 70% inhibition | Pseudomonas oleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
2-aminomuconate | pH 6.6, 25°C | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16600 | - |
6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
100000 | - |
gel filtration and PAGE | Pseudomonas oleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | P81593 | - |
- |
Pseudomonas oleovorans JS45 | P81593 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.8 | - |
pH 6.6, 25°C | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate + H2O | the deamination reaction is irreversible under the assay conditions | Pseudomonas oleovorans | 4-oxalocrotonate + NH3 | enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate | ir | |
2-aminomuconate + H2O | the deamination reaction is irreversible under the assay conditions | Pseudomonas oleovorans JS45 | 4-oxalocrotonate + NH3 | enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate | ir | |
additional information | no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid | Pseudomonas oleovorans | ? | - |
? | |
additional information | no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid | Pseudomonas oleovorans JS45 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 16600, SDS-PAGE | Pseudomonas oleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
stable at room temperature for 3 days | Pseudomonas oleovorans |
60 | - |
5 min, 23% residual activity | Pseudomonas oleovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
208 | - |
2-aminomuconate | pH 6.6, 25°C | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Pseudomonas oleovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8.8 | room temperature, stable for at least 3 h | Pseudomonas oleovorans |