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Literature summary for 3.5.99.10 extracted from

  • Miyakawa, T.; Hatano, K.I.; Lee, W.C.; Kato, Y.; Sawano, Y.; Yumoto, F.; Nagata, K.; Tanokura, M.
    Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7 (2005), Acta Crystallogr. Sect. F, 61, 828-830.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-iminobutanoate + H2O Sulfurisphaera tokodaii
-
 2-oxobutanoate + NH3
-
 ?
2-iminobutanoate + H2O Sulfurisphaera tokodaii 7
-
 2-oxobutanoate + NH3
-
 ?
2-iminopropanoate + H2O Sulfurisphaera tokodaii
-
 pyruvate + NH3
-
 ?
2-iminopropanoate + H2O Sulfurisphaera tokodaii 7
-
 pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q973T6
-
-
Sulfurisphaera tokodaii 7 Q973T6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Superdex 75 gel filtration Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-iminobutanoate + H2O
-
 Sulfurisphaera tokodaii 2-oxobutanoate + NH3
-
 ?
2-iminobutanoate + H2O
-
 Sulfurisphaera tokodaii 7 2-oxobutanoate + NH3
-
 ?
2-iminopropanoate + H2O
-
 Sulfurisphaera tokodaii pyruvate + NH3
-
 ?
2-iminopropanoate + H2O
-
 Sulfurisphaera tokodaii 7 pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
ST0811
-
 Sulfurisphaera tokodaii