Any feedback?
Literature summary for 3.5.5.8 extracted from
- Cloning and expression of a gene encoding a novel thermostable thiocyanate-degrading enzyme from a mesophilic Alphaproteobacteria strain THI201 (2013), Microbiology, 159, 2294-2302.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiocyanate | maximum activity at 20-40 mM thiocyanate | Alphaproteobacteria |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta-gami B and BL21(DE3) cells | Alphaproteobacteria |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Alphaproteobacteria |
| 50267 | - |
x * 50267, calculated from amino acid sequence | Alphaproteobacteria |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiocyanate + H2O | Alphaproteobacteria | - |
carbonyl sulfide + NH3 + HO- | - |
? | |
| thiocyanate + H2O | Alphaproteobacteria THI201 | - |
carbonyl sulfide + NH3 + HO- | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alphaproteobacteria | - |
- |
- |
| Alphaproteobacteria THI201 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxyapatite column chromatography (native enzyme) and glutathione Sepharose column chromatography (recombinant enzyme). The activity of the enzyme purified at room temperature is 1.4times that of the enzyme purified at 4°C | Alphaproteobacteria |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, recombinant enzyme, 1 week, complete loss of activity (72% of the original activity is recovered by the heat treatment of 60°C for 15 min) | Alphaproteobacteria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiocyanate + H2O | - |
Alphaproteobacteria | carbonyl sulfide + NH3 + HO- | - |
? | |
| thiocyanate + H2O | - |
Alphaproteobacteria THI201 | carbonyl sulfide + NH3 + HO- | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Alphaproteobacteria |
| ? | x * 50267, calculated from amino acid sequence | Alphaproteobacteria |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SCNase | - |
Alphaproteobacteria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Alphaproteobacteria |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Alphaproteobacteria |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
incubating recombinant enzyme on ice for 3 h reduces the enzyme activity to 2% of the original activity and subsequent heating at 60°C for 15 min returns the activity to the original level | Alphaproteobacteria |
| 30 | 80 | the activity of the recombinant enzyme is completely retained after heat treatment at temperatures from 30 to 70°C, but is decreased by heating at 80°C for 5 min to 80%, to 60% at 90°C, and to 50% at 100°C | Alphaproteobacteria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Alphaproteobacteria |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
