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Literature summary for 3.5.5.8 extracted from

  • Yamanaka, Y.; Arakawa, T.; Watanabe, T.; Namima, S.; Sato, M.; Hori, S.; Ohtaki, A.; Noguchi, K.; Katayama, Y.; Yohda, M.; Odaka, M.
    Two arginine residues in the substrate pocket predominantly control the substrate selectivity of thiocyanate hydrolase (2013), J. Biosci. Bioeng., 116, 22-27.
    View publication on PubMed
Search for more literature for 3.5.5.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-AI(DE3) cells Thiobacillus thioparus

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant R136W, hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) and 1.4 M sodium potassium tartrate Thiobacillus thioparus

Protein Variants

Protein Variants Comment Organism
R136W the mutant of the gamma subunit shows no thiocyanate hydrolysis activity but does catalyze the hydration of nitriles Thiobacillus thioparus
R90F the mutant of the beta subunit shows no thiocyanate hydrolysis activity but does catalyze the hydration of nitriles Thiobacillus thioparus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 Methacrylonitrile wild type enzyme, at pH 7.5 and 27°C Thiobacillus thioparus
93
-
 Methacrylonitrile mutant enzyme R90F, at pH 7.5 and 27°C Thiobacillus thioparus
160
-
 Methacrylonitrile mutant enzyme R136W, at pH 7.5 and 27°C Thiobacillus thioparus

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ contains cobalt Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiocyanate + H2O Thiobacillus thioparus
-
 carbonyl sulfide + NH3 + HO-
-
 ?
thiocyanate + H2O Thiobacillus thioparus THI115
-
 carbonyl sulfide + NH3 + HO-
-
 ?

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus O66187 and O66186 and O66188 O66187 subunit alpha, O66186 subunit beta, O66188 subunit gamma
-
Thiobacillus thioparus THI115 O66187 and O66186 and O66188 O66187 subunit alpha, O66186 subunit beta, O66188 subunit gamma
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methacrylonitrile + H2O
-
 Thiobacillus thioparus ?
-
 ?
methacrylonitrile + H2O
-
 Thiobacillus thioparus THI115 ?
-
 ?
thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + HO-
-
 ?
thiocyanate + H2O
-
 Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + HO-
-
 ?

Synonyms

Synonyms Comment Organism
SCNase
-
 Thiobacillus thioparus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 Methacrylonitrile mutant enzyme R136W, at pH 7.5 and 27°C Thiobacillus thioparus
2.1
-
 Methacrylonitrile mutant enzyme R90F, at pH 7.5 and 27°C Thiobacillus thioparus
57
-
 Methacrylonitrile wild type enzyme, at pH 7.5 and 27°C Thiobacillus thioparus