Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
azide | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
Borate | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
Br- | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
Cl- | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
Cyanate | significant inhibition | Thiohalophilus thiocyanatoxydans | |
nitrate | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
nitrite | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans | |
sulfite | inhibitory at 20 mM | Thiohalophilus thiocyanatoxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | contains 0.64 atoms Co2+ per heterotrimer | Thiohalophilus thiocyanatoxydans | |
Fe2+ | contains 0.41 atoms Fe2+ per heterotrimer | Thiohalophilus thiocyanatoxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration | Thiohalophilus thiocyanatoxydans |
19000 | - |
2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration | Thiohalophilus thiocyanatoxydans |
29000 | - |
2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration | Thiohalophilus thiocyanatoxydans |
130000 | - |
gel filtration | Thiohalophilus thiocyanatoxydans |
140000 | - |
SDS-PAGE | Thiohalophilus thiocyanatoxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiohalophilus thiocyanatoxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Mono Q GI column chromatography, and Superdex 200 gel filtration | Thiohalophilus thiocyanatoxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
crude extract, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5 | Thiohalophilus thiocyanatoxydans |
3.3 | - |
after 110fold purification, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5 | Thiohalophilus thiocyanatoxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | does not use thiosulfate as a substrate | Thiohalophilus thiocyanatoxydans | ? | - |
? | |
thiocyanate + H2O | - |
Thiohalophilus thiocyanatoxydans | carbonyl sulfide + NH3 + OH- | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | 2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration | Thiohalophilus thiocyanatoxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Thiohalophilus thiocyanatoxydans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Cyanate | - |
Thiohalophilus thiocyanatoxydans |