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Literature summary for 3.5.5.8 extracted from

  • Katayama, Y.; Matsushita, Y.; Kaneko, M.; Kondo, M.; Mizuno, T.; Nyunoya, H.
    Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase (1998), J. Bacteriol., 180, 2583-2589.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE Thiobacillus thioparus
23000
-
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE Thiobacillus thioparus
32000
-
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiocyanate + H2O Thiobacillus thioparus
-
carbonyl sulfide + NH3 + OH-
-
?

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiocyanate + H2O
-
Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
?

Subunits

Subunits Comment Organism
hexamer 2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE Thiobacillus thioparus