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Literature summary for 3.5.5.7 extracted from

  • Novikov, A.; Riabchenko, L.; Leonova, T.; Larikova, G.; Lavrov, K.; Glinskii, S.; Yanenko, A.
    Bacterial strain Alcaligenes denitrificans C-32 containing two nitrilases with different substrate specificities (2017), Appl. Biochem. Microbiol., 53, 786-791 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Achromobacter denitrificans

Organism

Organism UniProt Comment Textmining
Achromobacter denitrificans A0A286S9Z8
-
-
Achromobacter denitrificans A0A291PNG0
-
-
Achromobacter denitrificans C-32 A0A286S9Z8
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Achromobacter denitrificans C-32 A0A291PNG0
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + 2 H2O
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Achromobacter denitrificans acrylic acid + NH3
-
?
acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans acrylic acid + NH3
-
?
acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans C-32 acrylic acid + NH3
-
?
acrylonitrile + 2 H2O
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Achromobacter denitrificans C-32 acrylic acid + NH3
-
?
adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3
-
?
adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3
-
?
adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3
-
?
adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3
-
?
benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3
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?
benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3
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?
benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3
-
?
benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3
-
?
butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3
-
?
butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3
-
?
butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3
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?
butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3
-
?
phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3
-
?
phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3
-
?
phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3
-
?
phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3
-
?

Synonyms

Synonyms Comment Organism
nitC1
-
Achromobacter denitrificans
nitC2
-
Achromobacter denitrificans