Literature summary for 3.5.5.7 extracted from

  • Wang, H.; Li, G.; Li, M.; Wei, D.; Wang, X.
    A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization (2014), World J. Microbiol. Biotechnol., 30, 245-252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1% residual activity at 5 mM Cereibacter sphaeroides
Ca2+ 60% residual activity at 5 mM Cereibacter sphaeroides
Co2+ 75% residual activity at 5 mM Cereibacter sphaeroides
Cu2+ 43% residual activity at 5 mM Cereibacter sphaeroides
EDTA 63% residual activity at 5 mM Cereibacter sphaeroides
Fe2+ 44% residual activity at 5 mM Cereibacter sphaeroides
Mg2+ 93% residual activity at 5 mM Cereibacter sphaeroides
Ni2+ 87% residual activity at 5 mM Cereibacter sphaeroides
Zn2+ 19% residual activity at 5 mM Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
73.1
-
3-Cyanopyridine at pH 7.0 and 30°C Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
14 * 40000, SDS-PAGE Cereibacter sphaeroides
560000
-
gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides G5DDB2
-
-
Cereibacter sphaeroides LHS-305 G5DDB2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile Cereibacter sphaeroides ?
-
?
additional information no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile Cereibacter sphaeroides LHS-305 ?
-
?
4-chlorobutyronitrile + H2O 135% activity compared to 3-cyanopyridine Cereibacter sphaeroides 4-chlorobutyrate + NH3
-
?
4-chlorobutyronitrile + H2O 135% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 4-chlorobutyrate + NH3
-
?
succinonitrile + H2O 66.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides succinate + NH3
-
?
succinonitrile + H2O 66.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 succinate + NH3
-
?
benzonitrile + 2 H2O 55.4% activity compared to 3-cyanopyridine Cereibacter sphaeroides benzoate + NH3
-
?
benzonitrile + 2 H2O 55.4% activity compared to 3-cyanopyridine Cereibacter sphaeroides LHS-305 benzoate + NH3
-
?
3-cyanopyridine + 2 H2O 100% activity Cereibacter sphaeroides pyridine 3-carboxylic acid + NH3
-
?
3-cyanopyridine + 2 H2O 100% activity Cereibacter sphaeroides LHS-305 pyridine 3-carboxylic acid + NH3
-
?
acrylonitrile + H2O 25.9% activity compared to 3-cyanopyridine Cereibacter sphaeroides acrylate + NH3
-
?
1,4-dicyanobutane + H2O 114.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides ?
-
?
1,8-dicyanooctane + H2O 114.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides ?
-
?
2-methylglutaronitrile + 4 H2O 23.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides 2-methylglutarate + 2 NH3
-
?
3-phenylpropionitrile + H2O 147.3% activity compared to 3-cyanopyridine Cereibacter sphaeroides 3-phenylpropanoate + NH3
-
?
dodecanenitrile + H2O 13.5% activity compared to 3-cyanopyridine Cereibacter sphaeroides dodecanoate + NH3
-
?
fumaronitrile + H2O 209.8 activity compared to 3-cyanopyridine Cereibacter sphaeroides fumarate + NH3
-
?
pentanenitrile + H2O 62% activity compared to 3-cyanopyridine Cereibacter sphaeroides pentanoate + NH3
-
?
thiophene-3-carbonitrile + H2O 51.2% activity compared to 3-cyanopyridine Cereibacter sphaeroides ?
-
?

Subunits

Subunits Comment Organism
homotetradecamer 14 * 40000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cereibacter sphaeroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 about 55% activity at 30°C, about 80% activity at 35°C, 100% activity at 40°C, about 70% activity at 45°C, and about 30% activity at 50°C Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 60% activity at pH 6.0 and 80% activity at pH 8.0 in 10 mM sodium phosphate Cereibacter sphaeroides