Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzonitrile | - |
Rhodococcus rhodochrous |
Application | Comment | Organism |
---|---|---|
industry | the enzyme is engineered for the commercial production of 3-hydroxyvaleric acid | Acidovorax facilis |
industry | the high chemical specificity and frequent enantioselectivity of nitrilases makes them attractive biocatalysts for the production of fine chemicals and pharmaceutical intermediates. Nitrilases are also used in the treatment of toxic industrial effluent and cyanide remediation | Rhodococcus rhodochrous |
Protein Variants | Comment | Organism |
---|---|---|
F168V/L201N | the mutant shows increased activity and specificity compared to the wild type enzyme | Acidovorax facilis |
L201Q | the mutant shows increased activity and specificity compared to the wild type enzyme | Acidovorax facilis |
T210G | inactive | Acidovorax facilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29800 | - |
2 * 29800 | Pyrococcus abyssi |
38000 | - |
x * 38000 | Comamonas testosteroni |
40000 | - |
10 * 40000 | Synechocystis sp. |
40000 | - |
14 * 40000 | Acidovorax facilis |
40000 | - |
x * 40000, this enzyme exists as an inactive dimer which oligomerizes to form active spirals in the presence of benzonitrile or an autolytic cleavage of the C-terminus | Rhodococcus rhodochrous |
41000 | - |
- |
Pseudomonas sp. |
41000 | - |
14 * 41000 or 14 * 43000 | Acinetobacter sp. |
41000 | - |
14 * 41000 or 16 * 41000 | Rhodococcus rhodochrous |
43000 | - |
14 * 41000 or 14 * 43000 | Acinetobacter sp. |
60000 | - |
- |
Pyrococcus abyssi |
390000 | - |
- |
Synechocystis sp. |
570000 | - |
- |
Acidovorax facilis |
580000 | - |
- |
Acinetobacter sp. |
650000 | - |
- |
Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax facilis | - |
- |
- |
Acidovorax facilis 72W | - |
- |
- |
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. AK226 | - |
- |
- |
Comamonas testosteroni | Q59329 | - |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. S1 | - |
- |
- |
Pyrococcus abyssi | - |
- |
- |
Rhodococcus rhodochrous | Q02068 | - |
- |
Rhodococcus rhodochrous | Q03217 | - |
- |
Rhodococcus rhodochrous J1 | Q03217 | - |
- |
Rhodococcus rhodochrous K22 | Q02068 | - |
- |
Synechocystis sp. | Q55949 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | - |
Rhodococcus rhodochrous | nicotinic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | - |
Rhodococcus rhodochrous J1 | nicotinic acid + NH3 | - |
? | |
acrylonitrile + H2O | 100% activity | Pseudomonas sp. | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 128% activity compared to benzonitrile | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 144% activity compared to racemic Ibu-CN | Acinetobacter sp. | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | the enzyme shows higher activity with acrylonitrile as with benzonitrile | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 100% activity | Pseudomonas sp. S1 | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 144% activity compared to racemic Ibu-CN | Acinetobacter sp. AK226 | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | the enzyme shows higher activity with acrylonitrile as with benzonitrile | Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 128% activity compared to benzonitrile | Rhodococcus rhodochrous J1 | acrylic acid + NH3 | - |
? | |
adiponitrile + H2O | 100% activity | Comamonas testosteroni | adipic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Synechocystis sp. | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 3.8% activity compared to acrylonitrile | Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 4% activity compared to adiponitrile | Comamonas testosteroni | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 4.6% activity compared to fumaronitrile | Acidovorax facilis | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 94% activity compared to racemic Ibu-CN | Acinetobacter sp. | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 3.8% activity compared to acrylonitrile | Pseudomonas sp. S1 | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 94% activity compared to racemic Ibu-CN | Acinetobacter sp. AK226 | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous K22 | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 4.6% activity compared to fumaronitrile | Acidovorax facilis 72W | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 100% activity | Rhodococcus rhodochrous J1 | benzoic acid + NH3 | - |
? | |
fumaronitrile + H2O | 100% activity | Acidovorax facilis | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | 12000% activity compared to benzonitrile | Synechocystis sp. | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | 100% activity | Acidovorax facilis 72W | fumaric acid + NH3 | - |
? | |
malonitrile + H2O | - |
Pyrococcus abyssi | malonic acid + NH3 | - |
? | |
racemic Ibu-CN + 2 H2O | 100% activity | Acinetobacter sp. | ibuprofen + NH3 | - |
? | |
racemic Ibu-CN + 2 H2O | 100% activity | Acinetobacter sp. AK226 | ibuprofen + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000 | Comamonas testosteroni |
? | x * 40000, this enzyme exists as an inactive dimer which oligomerizes to form active spirals in the presence of benzonitrile or an autolytic cleavage of the C-terminus | Rhodococcus rhodochrous |
homodecamer | 10 * 40000 | Synechocystis sp. |
homodimer | 2 * 29800 | Pyrococcus abyssi |
homotetradecamer | 14 * 40000 | Acidovorax facilis |
homotetradecamer | 14 * 41000 or 14 * 43000 | Acinetobacter sp. |
homotetradecamer or homohexacecamer | 14 * 41000 or 16 * 41000 | Rhodococcus rhodochrous |
monomer | 1 * 41000 | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Comamonas testosteroni |
45 | - |
- |
Rhodococcus rhodochrous |
50 | - |
- |
Acinetobacter sp. |
50 | - |
- |
Synechocystis sp. |
50 | - |
- |
Rhodococcus rhodochrous |
65 | - |
- |
Acidovorax facilis |
100 | - |
- |
Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rhodococcus rhodochrous |
7 | - |
- |
Synechocystis sp. |
7 | - |
- |
Comamonas testosteroni |
7.4 | - |
- |
Pyrococcus abyssi |
7.6 | - |
- |
Rhodococcus rhodochrous |
8 | - |
- |
Acinetobacter sp. |
8 | 9 | - |
Acidovorax facilis |