Literature summary for 3.5.5.7 extracted from

  • Kim, J.S.; Tiwari, M.K.; Moon, H.J.; Jeya, M.; Ramu, T.; Oh, D.K.; Kim, I.W.; Lee, J.K.
    Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5 (2009), Appl. Microbiol. Biotechnol., 83, 273-283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 8% residual activity Pseudomonas fluorescens
Cu2+ 1 mM, no residual activity Pseudomonas fluorescens
Hg2+ 1 mM, 10% residual activity Pseudomonas fluorescens
N-ethylmaleimide 0.1 mM, 48% residual activity Pseudomonas fluorescens
pyridoxal 5'-phosphate 0.1 mM, 30% residual activity Pseudomonas fluorescens
Zn2+ 1 mM, no residual activity Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24
-
glutaronitrile pH 7.0, 30°C Pseudomonas fluorescens
2 3 Fumaronitrile pH 7.0, 30°C Pseudomonas fluorescens
23.3
-
acrylonitrile pH 7.0, 30°C Pseudomonas fluorescens
23.8
-
Benzonitrile pH 7.0, 30°C Pseudomonas fluorescens
25.3
-
sebaconitrile pH 7.0, 30°C Pseudomonas fluorescens
43.9
-
Propionitrile pH 7.0, 30°C Pseudomonas fluorescens
17.9
-
Succinonitrile pH 7.0, 30°C Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
138000
-
gel filtration Pseudomonas fluorescens
33000
-
4 * 33000, SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q4KCL8
-
-
Pseudomonas fluorescens Pf-5 Q4KCL8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
substrate succinonitrile Pseudomonas fluorescens
1.9
-
substrate fumaronitrile Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens ?
-
?
additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens Pf-5 ?
-
?
acrylonitrile + H2O
-
Pseudomonas fluorescens acrylic acid + NH3
-
?
acrylonitrile + H2O
-
Pseudomonas fluorescens Pf-5 acrylic acid + NH3
-
?
fumaronitrile + H2O
-
Pseudomonas fluorescens fumaric acid + NH3
-
?
fumaronitrile + H2O
-
Pseudomonas fluorescens Pf-5 fumaric acid + NH3
-
?
propionitrile + H2O
-
Pseudomonas fluorescens propionate + NH3
-
?
propionitrile + H2O
-
Pseudomonas fluorescens Pf-5 propionate + NH3
-
?
succinonitrile + H2O
-
Pseudomonas fluorescens succinate + NH3
-
?
succinonitrile + H2O
-
Pseudomonas fluorescens Pf-5 succinate + NH3
-
?
glutaronitrile + H2O
-
Pseudomonas fluorescens glutarate + NH3
-
?
sebaconitrile + H2O
-
Pseudomonas fluorescens ? + NH3
-
?
benzonitrile + 2 H2O
-
Pseudomonas fluorescens benzoate + NH3
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
NitPf5
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
50% of initial activity after 180 min Pseudomonas fluorescens
65
-
50% of initial activity after 45 min Pseudomonas fluorescens
30
-
50% of initial activity after 30 h Pseudomonas fluorescens
45
-
50% of initial activity after 300 min Pseudomonas fluorescens
16
-
50% of initial activity after 80 h Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
sebaconitrile pH 7.0, 30°C Pseudomonas fluorescens
0.17
-
Propionitrile pH 7.0, 30°C Pseudomonas fluorescens
8.8
-
Succinonitrile pH 7.0, 30°C Pseudomonas fluorescens
9.2
-
Fumaronitrile pH 7.0, 30°C Pseudomonas fluorescens
5
-
glutaronitrile pH 7.0, 30°C Pseudomonas fluorescens
0.22
-
Benzonitrile pH 7.0, 30°C Pseudomonas fluorescens
0.33
-
acrylonitrile pH 7.0, 30°C Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas fluorescens

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas fluorescens isoelectric focusing and calculated
-
5.4