Literature summary for 3.5.5.7 extracted from

  • Luo, H.; Fan, L.; Chang, Y.; Ma, J.; Yu, H.; Shen, Z.
    Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli (2008), Appl. Biochem. Biotechnol., 160, 393-400.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous tg1-A6
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O best substrate Rhodococcus rhodochrous acrylic acid + NH3
-
?
acrylonitrile + H2O best substrate Rhodococcus rhodochrous tg1-A6 acrylic acid + NH3
-
?
iminodiacetonitrile + 2 H2O 25% of the activity with acrylonitrile Rhodococcus rhodochrous iminodiacetic acid + 2 NH3
-
?
iminodiacetonitrile + 2 H2O 25% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 iminodiacetic acid + 2 NH3
-
?
4-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous 4-pyridinecarboxylic acid + NH3
-
?
4-cyanopyridine + 2 H2O 50% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 4-pyridinecarboxylic acid + NH3
-
?
glycolonitrile + H2O 40% of the activity with acrylonitrile Rhodococcus rhodochrous ammonium glycolate + ?
-
?
glycolonitrile + H2O 40% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 ammonium glycolate + ?
-
?
aminoacetonitrile + H2O 30% of the activity with acrylonitrile Rhodococcus rhodochrous aminoacetic acid + NH3
-
?
aminoacetonitrile + H2O 30% of the activity with acrylonitrile Rhodococcus rhodochrous tg1-A6 aminoacetic acid + NH3
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Rhodococcus rhodochrous