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Literature summary for 3.5.5.4 extracted from
- Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development (2007), Mol. Plant Pathol., 8, 623-637.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Brassica rapa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
3-Cyano-L-alanine | in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C | Brassica rapa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa | - |
cultivar Natsumaki | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration | Brassica rapa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Brassica rapa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyano-L-alanine + H2O | - |
Brassica rapa | L-aspartate + NH3 | - |
? | |
| additional information | indole-3-acetonitrile is no substrate for NIT-T4 | Brassica rapa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NIT-T4 | - |
Brassica rapa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.4 | - |
3-Cyano-L-alanine | in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C | Brassica rapa | |
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Release 2023.1 (January 2023)
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