Any feedback?
Literature summary for 3.5.5.4 extracted from
- Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog (2006), Plant Mol. Biol., 61, 111-122.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.18 | - |
3-Cyano-L-alanine | pH 8.0, 37°C | Lupinus angustifolius |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Lupinus angustifolius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus angustifolius | Q3LRV4 | isoform LaNIT4B, in addition to nitrilase activity, enzyme shows cyanoalanine hydratase activity | - |
| Lupinus angustifolius | Q5QGZ8 | isoform LaNIT4A, in addition to nitrilase activity, enzyme shows cyanoalanine hydratase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus angustifolius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.048 | - |
substrate phenyl-3-propionic acid, pH 8.0, 37°C | Lupinus angustifolius |
| 0.1 | - |
substrate phenyl-3-propionic acid, pH 8.0, 37°C | Lupinus angustifolius |
| 143.7 | - |
substrate 3-cyano-L-alanine, pH 8.0, 37°C | Lupinus angustifolius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyano-L-alanine + H2O | - |
Lupinus angustifolius | L-aspartate + NH3 | enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate | ? | |
| 3-cyano-L-alanine + H2O | - |
Lupinus angustifolius | L-aspartate + NH3 | enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate | ? | |
| additional information | no substrate: indole-3-acetonitrile | Lupinus angustifolius | ? | - |
? | |
| phenyl-3-propionitrile + H2O | - |
Lupinus angustifolius | phenyl-3-propionic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Lupinus angustifolius |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
