BRENDA - Enzyme Database
show all sequences of 3.5.5.1

Clostridium thermocellum nitrilase expression and surface display on Bacillus subtilis spores

Chen, H.; Zhang, T.; Sun, T.; Ni, Z.; Le, Y.; Tian, R.; Chen, Z.; Zhang, C.; J. Mol. Microbiol. Biotechnol. 25, 381-387 (2015) View publication on PubMed

Data extracted from this reference:

Application
Application
Commentary
Organism
industry
spore surface display of nitrilases is an effective approach for enzyme immobilization in biochemical engineering on an industrial scale
Hungateiclostridium thermocellum
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed, identified and displayed on the surface of Bacillus subtilis spores by using the spore coat protein G of Bacillus subtilis as an anchoring motif
Hungateiclostridium thermocellum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE
Hungateiclostridium thermocellum
Organism
Organism
UniProt
Commentary
Textmining
Hungateiclostridium thermocellum
A3DHT2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Hungateiclostridium thermocellum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-cyanopyridine + H2O
-
757454
Hungateiclostridium thermocellum
pyridine 3-carboxylic acid + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Hungateiclostridium thermocellum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Hungateiclostridium thermocellum
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
30°C: about 35% of maximal activity, 50°C: about 55% of maximal activity
Hungateiclostridium thermocellum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Hungateiclostridium thermocellum
Application (protein specific)
Application
Commentary
Organism
industry
spore surface display of nitrilases is an effective approach for enzyme immobilization in biochemical engineering on an industrial scale
Hungateiclostridium thermocellum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed, identified and displayed on the surface of Bacillus subtilis spores by using the spore coat protein G of Bacillus subtilis as an anchoring motif
Hungateiclostridium thermocellum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE
Hungateiclostridium thermocellum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hungateiclostridium thermocellum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-cyanopyridine + H2O
-
757454
Hungateiclostridium thermocellum
pyridine 3-carboxylic acid + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Hungateiclostridium thermocellum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Hungateiclostridium thermocellum
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
30°C: about 35% of maximal activity, 50°C: about 55% of maximal activity
Hungateiclostridium thermocellum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Hungateiclostridium thermocellum
Other publictions for EC 3.5.5.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752724
Jez
Plant nitrilase A new job fo ...
Arabidopsis thaliana
Biochem. J.
476
1105-1107
2019
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2
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5
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2
2
-
-
-
756271
Liu
Cloning, expression, and char ...
Pannonibacter carbonis, Pannonibacter carbonis Q4.6
Biotechnol. Lett.
41
583-589
2019
8
1
1
-
-
-
18
1
-
5
-
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7
-
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1
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19
-
1
1
1
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1
1
1
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8
1
1
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18
-
1
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5
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1
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19
-
1
1
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1
1
1
-
-
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-
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-
756359
Yu
-
Improving the catalytic effic ...
Synechocystis sp. PCC 6803
Catal. Sci. Technol.
9
1504-1510
2019
-
1
1
-
16
-
-
5
-
-
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1
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1
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14
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4
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1
1
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16
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5
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1
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14
-
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-
4
-
-
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-
-
-
-
5
5
756955
Xu
Enhanced catalytic stability ...
Acidovorax facilis
Int. J. Biol. Macromol.
130
117-124
2019
-
1
-
-
-
2
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
1
2
2
-
1
2
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1
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2
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2
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-
1
-
1
2
2
-
1
2
-
-
-
-
-
-
-
-
755668
Sharma
Classifying nitrilases as ali ...
Aeribacillus pallidus, Pseudomonas oleovorans, Synechocystis sp. PCC 6803, Arenibacter latericius, Stappia stellulata, Maricaulis maris, Rhizobium leguminosarum bv. trifolii, Blastomyces dermatitidis, Morganella morganii subsp. morganii, Sphingobacterium thalpophilum, Rhodococcus aetherivorans, Pseudomonas sp. GM41, Pantoea sp. AS-PWVM4, Fodinicurvata sediminis, Thalassospira lucentensis, Cellulophaga algicola DSM 14237, Burkholderia sp. BT03, Rubellimicrobium mesophilum, Tomitella biformata, Pedobacter jeongneungensis, Flexithrix dorotheae, Sediminispirochaeta bajacaliforniensis, Niabella soli, Butyrivibrio sp. MC2021, Dyadobacter alkalitolerans, Maribacter antarcticus, Chryseobacterium sp. UNC8MFCol, Calidithermus chliarophilus, Desulfatibacillum aliphaticivorans, Parabacteroides gordonii, Pseudonocardia spinosispora, Marssonina brunnea, Runella slithyformis, Bordetella bronchiseptica, Microscilla marina, Scheffersomyces stipitis, Janthinobacterium sp. Marseille, Shewanella sediminis, Saccharomyces cerevisiae, Burkholderia cenocepacia, Thalassiosira pseudonana, Burkholderia multivorans, Geodermatophilus obscurus, Streptomyces albus, Nocardiopsis dassonvillei ATCC 23218, Arabidopsis thaliana, Bacillus sp. OxB-1, Pseudomonas entomophila, Methanosarcina mazei, Burkholderia multivorans CGD1, Shewanella sediminis HAW-EB3, Pseudomonas entomophila L48, Rubellimicrobium mesophilum DSM 19309, Maricaulis maris MCS10, Blastomyces dermatitidis ER-3, Marssonina brunnea MB_m1, Geodermatophilus obscurus ATCC 25078, Pseudomonas oleovorans CECT:5344, Saccharomyces cerevisiae RM11-1a, Morganella morganii subsp. morganii KT, Scheffersomyces stipitis ATCC 58785, Niabella soli DSM 19437, Rhizobium leguminosarum bv. trifolii WSM1325, Microscilla marina ATCC 23134, Streptomyces albus J1074, Methanosarcina mazei BAA-159
3 Biotech
8
68
2018
-
-
-
-
-
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-
-
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68
-
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49
49
-
-
-
755874
Zhang
Characterization of a new nit ...
Hoeflea phototrophica, Hoeflea phototrophica DFL-43
Appl. Microbiol. Biotechnol.
102
6047-6056
2018
-
1
1
-
-
-
10
1
-
-
-
-
6
7
-
-
1
-
-
-
-
-
16
-
-
1
1
4
1
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
10
-
1
-
-
-
-
6
-
-
1
-
-
-
-
16
-
1
1
4
1
1
1
-
-
-
-
-
-
1
1
756503
Woodward
Substrate specificity of plan ...
Capsella rubella
Commun. Biol.
1
186
2018
-
-
-
-
5
-
-
-
-
-
-
-
-
9
-
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2
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3
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5
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2
-
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-
-
-
-
756618
Xu
Engineering the residues on " ...
Acidovorax facilis, Acidovorax facilis ZJB09122
Enzyme Microb. Technol.
113
52-58
2018
-
-
1
-
7
-
-
-
-
-
-
-
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2
-
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1
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1
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7
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1
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756946
Liu
Significant improvement of th ...
Acidovorax facilis
Int. J. Biol. Macromol.
116
563-571
2018
1
1
1
-
1
-
3
2
-
1
1
-
-
3
-
-
1
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2
1
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2
1
2
2
1
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1
1
1
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1
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3
-
2
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1
1
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1
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2
1
2
1
2
2
1
-
-
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-
-
-
-
-
-
756207
Xue
Improving catalytic performan ...
uncultured organism
Bioprocess Biosyst. Eng.
40
1565-1572
2017
-
-
-
-
1
-
-
8
-
-
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1
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4
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1
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8
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1
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8
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-
4
-
-
-
-
8
-
-
-
-
-
-
-
-
8
8
756212
Dong
Significantly enhanced substr ...
Pseudomonas putida
Biores. Technol.
244
1104-1110
2017
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
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2
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1
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2
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-
-
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756683
Cabrera
Cloning, overexpression, and ...
Pyrococcus sp. MC-FB
Extremophiles
21
861-869
2017
2
-
1
-
-
-
4
1
-
-
1
-
-
8
-
-
1
-
-
-
1
-
12
1
1
1
1
1
-
1
1
-
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-
-
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2
-
1
-
-
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-
4
-
1
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1
-
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1
-
-
1
-
12
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
756827
Lehmann
Arabidopsis nitrilase 1 contr ...
Arabidopsis thaliana
Front. Plant Sci.
8
36
2017
-
-
-
-
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-
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6
-
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3
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1
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3
-
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-
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1
1
-
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-
755864
Vesela
Bringing nitrilase sequences ...
Aspergillus luchuensis, Aspergillus luchuensis IFO 4308
Appl. Microbiol. Biotechnol.
100
2193-2202
2016
-
-
-
-
-
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7
-
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1
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17
-
1
1
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1
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1
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17
-
1
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1
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-
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756238
Su
High-level production of Arth ...
Paenarthrobacter aurescens, Paenarthrobacter aurescens CYC705
Biotechnol. Appl. Biochem.
63
564-571
2016
-
2
1
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21
-
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2
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1
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2
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756289
Sonbol
Red Sea Atlantis II brine poo ...
uncultured Burkholderiaceae bacterium, Luteovulum sphaeroides, Luteovulum sphaeroides LHS-305
BMC Biotechnol.
16
14
2016
-
2
1
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-
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7
1
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1
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7
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1
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3
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2
2
1
8
1
1
1
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2
1
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7
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1
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1
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1
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3
-
2
1
8
1
1
1
-
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-
-
1
1
756766
Dennett
A new thermophilic nitrilase ...
Pyrococcus sp. M24D13
Front. Bioeng. Biotechnol.
4
0000
2016
-
-
-
-
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5
1
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1
1
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5
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1
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1
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6
1
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1
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1
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1
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5
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1
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1
1
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1
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1
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6
1
1
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1
-
1
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-
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-
-
-
-
1
1
757020
Badoei-Dalfard
Production and characterizati ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa RZ44
Iran. J. Biotechnol.
14
142-153
2016
-
1
-
-
-
-
9
-
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2
1
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2
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1
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17
1
-
1
1
1
-
1
1
1
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1
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-
-
-
-
-
10
-
-
-
2
1
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-
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1
-
-
-
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17
1
1
1
1
-
1
1
1
-
2
-
-
2
-
-
733521
Li
Improving the catalytic potent ...
Fusarium proliferatum, Fusarium proliferatum CA3-1
Bioprocess Biosyst. Eng.
38
189-197
2015
-
-
-
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1
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2
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6
-
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2
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1
1
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1
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2
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1
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2
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2
-
1
-
1
-
2
-
-
-
-
-
-
-
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-
746898
Martinkova
Cyanide hydratases and cyanid ...
Bacillus pumilus
Appl. Microbiol. Biotechnol.
99
8875-8882
2015
-
1
-
-
1
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5
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1
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1
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-
-
-
-
-
-
-
-
756840
Yusuf
Cloning and functional charac ...
Fusarium proliferatum, Fusarium proliferatum AUF-2
Funct. Integr. Genomics
15
413-424
2015
1
-
1
-
-
-
12
1
-
6
1
-
-
13
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1
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1
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22
1
-
1
1
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1
1
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1
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1
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-
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12
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1
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6
1
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1
-
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1
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22
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
757343
Cai
-
Characterization of a nitrila ...
Paenarthrobacter aurescens
J. Gen. Appl. Microbiol.
60
207-214
2015
-
-
1
-
-
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1
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2
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1
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1
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14
1
1
1
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-
1
1
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1
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-
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-
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1
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2
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1
-
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-
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14
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1
757454
Chen
Clostridium thermocellum nitr ...
Hungateiclostridium thermocellum
J. Mol. Microbiol. Biotechnol.
25
381-387
2015
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1
1
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1
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11
-
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1
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4
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1
1
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1
1
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1
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1
1
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1
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1
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4
-
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1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
758526
Ricano-Rodríguez
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Purification and characteriza ...
Trichoderma harzianum, Trichoderma harzianum VSL291
Turk. J. Biol.
39
248-257
2015
-
-
-
-
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14
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1
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2
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1
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-
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4
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1
1
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1
1
4
-
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-
-
-
-
-
-
14
-
-
1
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-
-
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-
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1
-
-
-
-
4
-
1
-
-
-
1
1
4
-
-
-
-
-
-
-
728981
Rucka
Expression control of nitrile ...
Rhodococcus erythropolis, Rhodococcus erythropolis A4
Antonie van Leeuwenhoek
105
1179-1190
2014
-
-
1
-
-
-
-
-
-
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1
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2
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1
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-
-
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11
1
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1
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1
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1
-
-
-
-
11
1
-
-
-
-
-
-
-
-
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-
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-
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-
729043
Duca
Characterization of a nitrilas ...
Pseudomonas sp.
Appl. Environ. Microbiol.
80
4640-4649
2014
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-
1
-
-
-
-
-
-
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1
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-
10
-
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1
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-
-
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1
1
1
1
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1
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1
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1
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-
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-
1
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1
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
734397
Cai
Characterization of a nitrilas ...
Paenarthrobacter aurescens, Paenarthrobacter aurescens CYC705
J. Gen. Appl. Microbiol.
60
207-214
2014
-
-
1
-
-
-
-
1
-
-
3
-
-
10
-
-
1
-
-
-
-
-
20
2
1
1
-
1
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
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1
-
-
-
-
20
2
1
-
1
1
1
1
-
-
-
-
-
-
1
1
734501
Zhu
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Characterization and functiona ...
Pseudomonas putida, Pseudomonas putida CGMCC3830
J. Mol. Catal. B
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175-183
2013
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1
-
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14
1
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2
2
-
2
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-
1
-
-
-
-
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21
1
-
1
1
-
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
14
-
1
-
-
2
2
-
-
-
1
-
-
-
-
21
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
718648
Baum
Conversion of sterically deman ...
Alcaligenes faecalis, Synechocystis sp., Rhodococcus sp., Pseudomonas fluorescens, Pseudomonas fluorescens EBC191, Alcaligenes faecalis ATCC 8750
Appl. Environ. Microbiol.
78
48-57
2012
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4
4
-
5
-
-
4
-
-
-
6
-
22
-
-
-
-
-
-
4
-
28
-
-
4
-
-
-
4
-
-
-
-
-
-
-
4
4
-
-
5
-
-
-
-
4
-
-
-
6
-
-
-
-
-
-
4
-
28
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
718670
Petrickova
Purification and characterizat ...
Talaromyces marneffei, Fusarium verticillioides, Talaromyces marneffei ATCC 18224
Appl. Microbiol. Biotechnol.
93
1553-1561
2012
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-
2
-
-
-
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13
-
-
-
13
-
23
-
-
2
-
-
-
4
-
19
-
-
2
-
7
-
2
-
8
-
-
-
-
-
-
2
-
-
-
-
-
-
-
13
-
-
-
13
-
-
-
2
-
-
4
-
19
-
2
-
7
-
2
-
8
-
-
-
-
-
-
-
720279
Liu
Biosynthesis of iminodiacetic ...
Acidovorax facilis
J. Mol. Microbiol. Biotechnol.
22
35-47
2012
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1
1
-
1
-
-
-
-
1
1
-
-
15
-
-
-
-
-
-
1
-
13
1
-
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
13
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
735075
Gong
Fungal His-tagged nitrilase fr ...
Fusarium proliferatum, Fusarium proliferatum CA3-1
PLoS ONE
7
e50622
2012
1
-
1
-
-
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13
1
-
1
2
2
1
6
-
-
1
-
-
-
-
-
21
2
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
13
-
1
-
1
2
2
1
-
-
1
-
-
-
-
21
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
710984
Bayer
A nitrilase from a metagenomic ...
uncultured bacterium
Appl. Microbiol. Biotechnol.
89
91-98
2011
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1
-
-
-
-
1
-
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1
-
-
3
-
-
1
-
-
1
-
-
6
1
2
1
-
3
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
6
1
1
-
3
-
1
1
-
-
-
-
-
-
1
1
718611
Yang
A new nitrilase-producing stra ...
Luteovulum sphaeroides, Luteovulum sphaeroides LHS-305
Appl. Biochem. Biotechnol.
165
1556-1567
2011
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1
-
-
-
-
1
1
-
-
-
-
-
4
-
-
-
-
-
1
1
-
8
-
-
1
-
3
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
1
-
8
-
1
-
3
-
1
-
-
-
2
-
-
2
-
-
719102
Zhang
Cloning and biochemical proper ...
Alcaligenes sp., Alcaligenes sp. ECU0401
Bioprocess Biosyst. Eng.
34
315-322
2011
1
-
1
-
-
-
13
1
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3
1
-
-
10
-
-
1
-
-
-
1
-
13
1
-
1
-
-
-
1
-
1
-
-
1
-
1
-
1
-
-
-
-
-
13
-
1
-
3
1
-
-
-
-
1
-
-
1
-
13
1
1
-
-
-
1
-
1
1
-
-
-
-
-
-
719103
Nageshwar
Optimization of nitrilase prod ...
Alcaligenes faecalis, Alcaligenes faecalis MTCC 10757
Bioprocess Biosyst. Eng.
34
515-523
2011
-
-
-
-
-
-
-
-
-
-
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28
-
8
-
-
-
-
-
1
-
-
30
-
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
28
-
-
-
-
-
1
-
-
30
-
1
1
1
-
1
1
-
-
1
-
-
1
-
-
719168
Kaplan
Heterologous expression, purif ...
Aspergillus niger, Aspergillus niger K10
BMC Biotechnol.
11
2
2011
-
-
1
-
-
1
-
-
-
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2
-
-
9
-
1
1
-
1
-
-
-
12
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
1
1
-
-
-
12
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710792
Gupta
-
Purification and characterizat ...
Rhodococcus rhodochrous, Rhodococcus fascians, Rhodococcus fascians MTCC-1531, Rhodococcus rhodochrous MTCC-291
Afr. J. Microbiol. Res.
4
1148-1153
2010
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-
-
-
-
-
-
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4
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-
-
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4
-
-
-
-
-
-
-
-
4
-
-
2
2
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
4
-
2
2
2
-
2
-
2
-
-
-
-
-
-
-
710981
Williamson
Structural and biochemical cha ...
Aeribacillus pallidus, Aeribacillus pallidus RAPc8 (NRRL: B-59396)
Appl. Microbiol. Biotechnol.
88
143-153
2010
-
-
-
-
-
-
-
-
-
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2
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15
-
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1
-
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711335
Vesela
Hydrolysis of benzonitrile her ...
Nocardia globerula, Rhodococcus rhodochrous, Rhodococcus sp., Rhodococcus sp. NDB 1165, Nocardia globerula NHB-2, Rhodococcus rhodochrous PA-34
Biodegradation
21
761-770
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
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30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711496
Kumar
Cross-linked enzyme aggregates ...
Pseudomonas putida, Pseudomonas putida MTCC 5110
Biores. Technol.
101
6856-6858
2010
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1
-
-
1
-
-
-
-
-
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6
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
711870
Schreiner
-
Directed evolution of Alcalige ...
Alcaligenes faecalis, Alcaligenes faecalis JM3
Enzyme Microb. Technol.
47
140-146
2010
-
-
-
-
10
-
-
-
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-
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2
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1
-
-
-
4
-
4
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
4
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
712015
Yeom
A positively charged amino aci ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous ATCC 33278
FEBS Lett.
584
106-110
2010
-
-
1
-
13
-
-
11
-
-
-
-
-
5
-
-
-
-
-
-
22
-
11
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
1
-
-
13
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
22
-
11
-
-
-
-
11
-
-
-
-
-
-
-
-
11
11
712161
Agerbirk
Complex metabolism of aromatic ...
Arabis hirsuta
Insect Biochem. Mol. Biol.
40
126-137
2010
-
-
-
-
-
-
-
-
-
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7
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-
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-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713440
Vejvoda
-
Purification and characterizat ...
Fusarium solani, Fusarium solani IMI196840, Fusarium solani O1
Process Biochem.
45
1115-1120
2010
1
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-
-
-
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15
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3
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10
-
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1
-
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-
1
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21
1
-
2
1
2
-
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
15
-
-
-
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3
-
-
-
-
1
-
-
1
-
21
1
2
1
2
-
2
2
-
-
-
-
-
-
-
-
713444
Zhang
-
Efficient production of (R)-(- ...
Alcaligenes sp., Alcaligenes sp. ECU0401
Process Biochem.
45
887-891
2010
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1
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1
-
1
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1
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3
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1
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1
-
2
1
-
1
1
1
1
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
1
1
1
1
1
1
1
-
-
-
-
-
-
1
1
720318
Ishikawa
-
Isoform-specific localization ...
Brassica rapa
J. Plant Growth Regul.
29
210-222
2010
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1
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-
-
-
-
-
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-
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1
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-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698295
Zhang
Mammalian nitrilase 1 homologu ...
Mus musculus
Int. Immunol.
21
691-703
2009
-
-
-
-
1
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-
-
-
-
-
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9
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1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
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-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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699724
Ankati
Asymmetric synthesis of both a ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
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74
1658-1662
2009
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1
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22
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-
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16
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-
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-
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-
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1
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-
16
-
-
-
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-
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-
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-
-
-
710966
Malandra
Continuous hydrolysis of 4-cya ...
Aspergillus niger, Fusarium solani, Fusarium solani O1, Aspergillus niger K10
Appl. Microbiol. Biotechnol.
85
277-284
2009
-
-
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27
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4
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2
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4
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-
2
-
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-
712260
Thuku
Microbial nitrilases: Versatil ...
Arthrobacter sp., Bradyrhizobium japonicum, Brassica napus, Fusarium solani, Rhodococcus rhodochrous, Rhodococcus sp., Fusarium oxysporum f. sp. melonis, Aspergillus niger, Arabidopsis thaliana, Aeribacillus pallidus, Bradyrhizobium japonicum USDA 110, Rhodococcus sp. NCIMB 11216, Fusarium solani IMI 196840, Fusarium solani O1, Aeribacillus pallidus Dac521, Rhodococcus rhodochrous ATCC 39484, Aspergillus niger K10, Rhodococcus sp. NCIMB 11215, Rhodococcus rhodochrous PA-34
J. Appl. Microbiol.
106
703-727
2009
1
7
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-
1
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23
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-
54
-
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-
49
13
1
11
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-
-
12
-
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-
1
7
-
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-
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1
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23
-
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-
49
13
11
-
-
-
12
-
-
-
-
-
-
-
-
-
712784
Winkler
-
Biocatalytic application of ni ...
Aspergillus niger, Fusarium solani, Fusarium solani O1, Aspergillus niger K10
J. Mol. Catal. B
59
243-247
2009
-
-
-
-
-
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-
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11
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2
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2
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38
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-
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2
-
2
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-
38
-
-
-
-
-
-
-
-
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-
-
-
-
685014
Yeom
A determinant residue of subst ...
Rhodococcus rhodochrous
Biochem. J.
415
401-407
2008
-
-
1
-
10
-
-
14
-
-
1
-
-
11
-
-
1
-
-
-
14
-
3
1
-
-
-
-
14
-
-
-
-
-
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-
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-
1
-
-
10
-
-
-
-
14
-
-
1
-
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-
1
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-
14
-
3
1
-
-
-
14
-
-
-
-
-
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-
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-
-
685635
Naik
Studies on the production of e ...
Pseudomonas putida, Pseudomonas putida MTCC 5110
Biores. Technol.
99
26-31
2008
-
-
-
-
-
-
-
-
-
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-
8
-
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2
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1
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-
1
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-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
688441
Vejvoda
-
Purification and characterizat ...
Fusarium solani, Fusarium solani O1
J. Mol. Catal. B
50
99-106
2008
-
-
-
-
-
-
15
3
-
1
2
-
12
7
-
-
1
-
-
1
-
2
21
1
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
15
-
3
-
1
2
-
12
-
-
1
-
1
-
2
21
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
694107
Banerjee
enantioselective nitrilase fro ...
Pseudomonas putida
Mol. Biotechnol.
41
35-41
2008
-
1
1
-
-
-
-
-
-
-
-
-
-
14
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695708
Luo
Gene cloning, overexpression, ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Appl. Biochem. Biotechnol.
160
393-400
2008
-
-
2
-
-
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
15
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
15
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
695813
Chmura
Utilization of arylaliphatic n ...
Halomonas nitrilicus
Appl. Microbiol. Biotechnol.
81
371-378
2008
-
-
-
-
-
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-
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-
13
-
-
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6
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-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696238
Barglow
Functional proteomic and struc ...
Mus musculus
Biochemistry
47
13514-13523
2008
-
-
1
1
6
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
-
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-
1
-
1
6
-
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-
1
-
-
-
-
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-
-
-
-
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-
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-
-
-
-
699360
Kaul
Predicting enzyme behavior in ...
Alcaligenes faecalis, Alcaligenes faecalis MTCC 126
J. Ind. Microbiol. Biotechnol.
35
713-720
2008
-
-
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14
2
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-
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2
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-
14
-
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684249
Zhu
-
Nitrilase-catalyzed selective ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
Adv. Synth. Catal.
349
1667-1670
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
22
-
-
-
-
-
-
-
-
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684250
Kaul
-
Cross-linked amorphous nitrila ...
Alcaligenes faecalis
Adv. Synth. Catal.
349
2167-2176
2007
-
-
1
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
684564
Zhu
Novel sensitive high-throughpu ...
Bacillus subtilis, Rhodococcus hoagii, Rhodococcus hoagii CCTCC.M.205114, Bacillus subtilis E9
Appl. Environ. Microbiol.
73
6053-6057
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
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-
-
-
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4
-
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-
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-
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685712
Wu
Protein engineering of Acidovo ...
Acidovorax facilis, Acidovorax facilis 72W
Biotechnol. Bioeng.
97
689-693
2007
-
-
1
-
7
-
-
-
-
-
1
-
-
16
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685715
Wu
Protein engineering of nitrila ...
Acidovorax facilis, Acidovorax facilis 72W
Biotechnol. Bioeng.
99
717-720
2007
-
-
-
-
15
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685730
Vejvoda
Biotransformation of heterocyc ...
Fusarium solani
Biotechnol. Lett.
29
1119-1124
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686105
He
-
Biocatalytic synthesis of (R)- ...
Alcaligenes sp., Alcaligenes sp. ECU0401
Chin. Chem. Lett.
18
677-680
2007
-
-
-
-
-
-
-
-
-
-
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3
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2
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686446
Rustler
-
Conversion of mandelonitrile a ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC191
Enzyme Microb. Technol.
40
598-606
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
686652
Thuku
Post-translational cleavage of ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous J1
FEBS J.
274
2099-2108
2007
-
-
1
-
-
-
-
-
-
-
3
-
-
23
-
1
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688087
Kriechbaumer
Maize nitrilases have a dual r ...
Zea mays
J. Exp. Bot.
58
4225-4233
2007
2
-
-
-
-
-
2
1
-
-
-
-
-
11
-
-
1
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
689084
Ishikawa
Molecular cloning of Brassica ...
Brassica rapa
Mol. Plant Pathol.
8
623-637
2007
-
-
1
-
-
-
-
5
-
-
-
-
-
10
-
-
1
-
-
1
-
-
6
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
1
-
-
6
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
689769
Jenrich
Evolution of heteromeric nitri ...
Sorghum bicolor
Proc. Natl. Acad. Sci. USA
104
18848-18853
2007
-
-
1
-
-
-
-
-
-
-
2
-
-
10
-
-
1
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667290
Banerjee
Enhancing the catalytic potent ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
72
77-87
2006
-
1
-
-
-
1
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668688
Vejvoda
Mild hydrolysis of nitriles by ...
Fusarium solani, Fusarium solani O1
Folia Microbiol. (Praha)
51
251-256
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
1
-
-
2
-
-
4
-
-
1
2
1
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
4
-
1
2
1
-
2
2
-
-
-
-
-
-
-
-
669903
Yildirim
-
Selective hydrolysis of the ni ...
Arabidopsis thaliana, Rhodococcus sp.
J. Mol. Catal. B
38
76-83
2006
-
2
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670462
Kamila
Unexpected stereorecognition i ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA 110
Org. Lett.
8
4429-4431
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
25
-
-
-
-
-
-
-
-
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684243
Fernandes
-
Nitrile hydratase activity of ...
Pseudomonas fluorescens, Pseudomonas fluorescens EBC 191
Adv. Synth. Catal.
348
2597-2603
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684594
Kaplan
Purification and characterizat ...
Aspergillus niger, Aspergillus niger K10
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685425
Holtze
Transformation of the herbicid ...
Pseudomonas putida, Pseudomonas fluorescens, Rhizobium sp., Pseudomonas putida 11388, Pseudomonas fluorescens 11387, Rhizobium sp. 11401
Biodegradation
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2006
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686039
Singh
Nitrilase and its application ...
Acinetobacter sp., Alcaligenes faecalis, Arabidopsis thaliana, Aeribacillus pallidus, Bacillus sp. (in: Bacteria), Comamonas testosteroni, Fusarium oxysporum, Fusarium solani, Hordeum vulgare, Acidovorax facilis, Klebsiella pneumoniae, Penicillium multicolor, Pseudomonas sp., Rhodococcus rhodochrous, Rhodococcus sp., Fusarium solani IMI196840, Acinetobacter sp. AK226, Bacillus sp. (in: Bacteria) OxB-1, Rhodococcus rhodochrous K22, Aeribacillus pallidus Dac521, Acidovorax facilis 72W
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Mukherjee
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688430
Yazbeck
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A metal ion-based method for t ...
Arabidopsis thaliana
J. Mol. Catal. B
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2006
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688432
Xie
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Arabidopsis thaliana
J. Mol. Catal. B
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668039
Mustacchi
The effect of whole cell immob ...
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Kabaivanova
Immobilization of cells with n ...
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670592
Dixon
Stress-induced protein S-gluta ...
Arabidopsis thaliana
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2005
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The signature amidase from Sul ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
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7
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10
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654314
Brady
Characterisation of nitrilase ...
Rhodococcus sp.
Appl. Microbiol. Biotechnol.
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2004
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651610
O'Reilly
The nitrilase family of CN hyd ...
Acinetobacter sp., Alcaligenes faecalis, Arabidopsis thaliana, Aeribacillus pallidus, Bacillus sp. (in: Bacteria), Brassica rapa, Comamonas testosteroni, Fusarium oxysporum, Fusarium solani, Hordeum vulgare, Acidovorax facilis, Klebsiella pneumoniae, Nocardia sp., Pseudomonas sp., Rhodococcus rhodochrous, Rhodococcus sp., Fusarium solani IMI196840, Rhodococcus sp. ATCC 39484, Acinetobacter sp. AK226, Bacillus sp. (in: Bacteria) OxB-1, Rhodococcus rhodochrous K22, Alcaligenes faecalis JM3, Aeribacillus pallidus Dac521, Acidovorax facilis 72W
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13
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Brenner
Catalysis in the nitrilase sup ...
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2002
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Vorwerk
Enzymatic characterization of ...
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Nagawasa
Nitrilase of Rhodococcus rhodo ...
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209744
Grsic-Rausch
Expression and localization of ...
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2000
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Almatawah
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Thermostable nitrilase catalys ...
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Almatawah
Characterization of an inducib ...
Aeribacillus pallidus
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8
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24
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Dhillon
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Transformation of aliphatic an ...
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1
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-
1
1
-
20
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
1
2
-
-
-
1
-
1
1
-
20
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
209756
Stalker
Cloning and expression in Esch ...
Klebsiella pneumoniae subsp. ozaenae
J. Bacteriol.
169
955-960
1987
-
-
1
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-
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-
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5
-
-
-
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1
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1
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1
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-
-
-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209757
McBride
Metabolism of the herbicide br ...
Klebsiella pneumoniae subsp. ozaenae
Appl. Environ. Microbiol.
52
325-330
1986
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-
-
-
-
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1
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5
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-
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1
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4
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1
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1
-
-
4
-
-
-
-
-
-
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-
-
-
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-
-
-
-
209758
Bandypadhyay
Purification and characterizat ...
Arthrobacter sp., Arthrobacter sp. J-1
Appl. Environ. Microbiol.
51
302-306
1986
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-
-
-
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5
2
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2
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4
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1
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1
2
-
8
1
2
2
-
2
-
2
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-
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5
-
2
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-
2
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1
-
1
2
-
8
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
209759
Kuwahara
-
Metabolism of aliphatic nitril ...
Fusarium solani
J. Ferment. Technol.
58
573-577
1980
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-
-
-
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1
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1
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1
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1
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1
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1
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-
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1
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-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
209760
Rausch
-
Partial purification of nitril ...
Brassica rapa subsp. pekinensis
Phytochemistry
19
747-750
1980
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-
-
-
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-
2
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-
2
-
1
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1
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2
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-
2
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-
1
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1
2
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-
2
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-
2
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1
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2
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-
2
-
-
-
1
-
1
2
-
-
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-
-
-
-
209761
Harper
Fungal degradation of aromatic ...
Fusarium solani
Biochem. J.
167
685-692
1977
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-
-
-
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5
1
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-
2
2
-
1
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-
1
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1
1
-
17
1
-
-
-
1
-
-
1
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-
1
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-
-
-
-
-
-
-
5
1
1
-
-
2
2
-
-
-
1
-
1
1
-
17
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
209762
Harper
Microbial metabolism of aromat ...
Nocardia sp.
Biochem. J.
165
309-319
1977
-
-
-
-
-
-
11
1
-
-
2
2
-
1
-
-
1
-
-
1
1
-
14
1
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
1
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-
2
2
-
-
-
1
-
1
1
-
14
1
-
-
-
-
1
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-
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-
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-
-
-
209763
Harper
Purification and properties of ...
Nocardia sp.
Biochem. Soc. Trans.
4
502-504
1976
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1
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1
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3
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
209764
Thimann
Nitrilase. I. Occurrence, prep ...
Hordeum vulgare, no activity in Allium cepa, no activity in Citrus limon, no activity in Croton sp., no activity in Cucumis sativus, no activity in Ginkgo biloba, no activity in Helianthus annuus, no activity in Heliconia sp., no activity in Impatiens balsamina, no activity in Iris sp., no activity in Kalanchoe blossfeldiana, no activity in Lilium sp., no activity in Solanum lycopersicum, no activity in Monstera deliciosa, no activity in Nerium oleander, no activity in Onoclea sensibilis, no activity in Pelargonium zonale, no activity in Pteridium aquilinum, no activity in Rosa sp., no activity in Tradescantia virginiana, no activity in Xanthium pennsylvanicum
Arch. Biochem. Biophys.
105
133-141
1964
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-
-
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-
6
1
1
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-
1
-
21
-
-
1
-
-
1
-
-
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
1
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
1
-
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-