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Literature summary for 3.5.5.1 extracted from
- Production and characterization of a nitrilase from Pseudomonas aeruginosa RZ44 and its potential for nitrile biotransformation (2016), Iran. J. Biotechnol., 14, 142-153 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | Pseudomonas aeruginosa RZ44 has the potential to be applied in the biotransformation of nitrile compounds | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 48% loss of activity | Pseudomonas aeruginosa |  |
| Co2+ | 1 mM, 84% loss of activity | Pseudomonas aeruginosa | |
| Cu2+ | 1 mM, 61% loss of activity | Pseudomonas aeruginosa | |
| DTT | 1 mM, 15% loss of activity | Pseudomonas aeruginosa | |
| EDTA | 1 mM, 7% loss of activity | Pseudomonas aeruginosa | |
| Fe3+ | 1 mM, 48% loss of activity | Pseudomonas aeruginosa | |
| H2O2 | 1 mM, 73% loss of activity | Pseudomonas aeruginosa | |
| Mn2+ | 1 mM, 59% loss of activity | Pseudomonas aeruginosa | |
| Zn2+ | 1 mM, 20% loss of activity; 1 mM, 24% loss of activity | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1 mM, 1.1fold increase in activity | Pseudomonas aeruginosa | |
| Mg2+ | 1 mM, 1.1fold increase in activity | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa RZ44 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-amino-benzonitrile + H2O | 88% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | 2-aminobenzoic acid + NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | 102% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | pyridine 3-carboxylic acid + NH3 | - |
? | |
| acetamide + H2O | 119% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | acetate + NH3 | - |
? | |
| acetonitrile + H2O | - |
Pseudomonas aeruginosa | acetic acid + NH3 | - |
? | |
| acrylamide + H2O | 101% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 107% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | 107% of the activity as compared to acetonitrile | Pseudomonas aeruginosa RZ44 | acrylic acid + NH3 | - |
? | |
| benzamide + H2O | 257% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | benzoic acid + NH3 | - |
? | |
| benzamide + H2O | 257% of the activity as compared to acetonitrile | Pseudomonas aeruginosa RZ44 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 94% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | benzoate + NH3 | - |
? | |
| benzonitrile + 2 H2O | 94% of the activity as compared to acetonitrile | Pseudomonas aeruginosa RZ44 | benzoate + NH3 | - |
? | |
| bis-acrylamide + H2O | 67% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | ? | - |
? | |
| malononitrile + H2O | 44% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | malonic acid + NH3 | - |
? | |
| malononitrile + H2O | 44% of the activity as compared to acetonitrile | Pseudomonas aeruginosa RZ44 | malonic acid + NH3 | - |
? | |
| N-methyl-propionamide + H2O | 161% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | ? | - |
? | |
| pyridine + H2O | 23% of the activity as compared to acetonitrile | Pseudomonas aeruginosa | ? | - |
? | |
| pyridine + H2O | 23% of the activity as compared to acetonitrile | Pseudomonas aeruginosa RZ44 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 60% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
highest stability at 40°C | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6.0: about 70% of maximal activity, pH 8.0: about 60% of maximal activity | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
highest activity at pH 7.0 | Pseudomonas aeruginosa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas aeruginosa | addition of Co2+, Mn2+ and Cu2+ to the grwoth medium of Pseudomonas aeruginosa RZ44 inhibits cell growth and enzyme production | down |
| Pseudomonas aeruginosa | nitrilase production increases in the pH range 5.0 to 7.0 and then starts decreasing. Addition of Mg2+, Fe2+ and Na+ to the grwoth medium of Pseudomonas aeruginosa RZ44 supports the biomass and nitrilase production | up |
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