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Literature summary for 3.5.5.1 extracted from

  • Xu, Z.; Huang, J.W.; Xia, C.J.; Zou, S.P.; Xue, Y.P.; Zheng, Y.G.
    Enhanced catalytic stability and reusability of nitrilase encapsulated in ethyleneamine-mediated biosilica for regioselective hydrolysis of 1-cyanocycloalkaneacetonitrile (2019), Int. J. Biol. Macromol., 130, 117-124 .
    View publication on PubMed
Search for more literature for 3.5.5.1

Application

Application Comment Organism
synthesis Nitrilase-catalyzed regioselective hydrolysis of 1-cyanocyclohexaneacetonitrile is a green and efficient approach for the preparation of 1-cyanocyclohexaneacetic acid, a key precursor for the synthesis of gabapentin. The enzyme ecapsulated in ethyleneamine-mediated biosilica an be used as a biocatalyst for the development of an efficient biotransformation process for the synthesis of 1-cyanocyclohexaneacetic acid Acidovorax facilis

General Stability

General Stability Organism
high recycling efficiency of enzyme ecapsulated in ethyleneamine-mediated biosilica. The residual activity of the enzyme ecapsulated in ethyleneamine-mediated biosilica retains 94.2% of its initial activity after 16 cycles of reaction. Even after 20 cycles, its residual activity keeps approximately 77.1% of its initial activity Acidovorax facilis
the enzyme ecapsulated in ethyleneamine-mediated biosilica has significantly improved temperature tolerance Acidovorax facilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
141
-
 1-cyanocyclohexaneacetonitrile 40°C, pH 7.0, soluble enzyme Acidovorax facilis
280
-
 1-cyanocyclohexaneacetonitrile 40°C, pH 7.0, enzyme ecapsulated in ethyleneamine-mediated biosilica Acidovorax facilis

Organism

Organism UniProt Comment Textmining
Acidovorax facilis Q1W2L4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-cyanocyclohexaneacetonitrile + H2O
-
 Acidovorax facilis 1-cyanocyclohexaneacetic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NitA
-
 Acidovorax facilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 soluble and enzyme ecapsulated in ethyleneamine-mediated biosilica Acidovorax facilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 55% of maximal activity, 60°C: about 65% of maximal activity, soluble enzyme Acidovorax facilis
20 60 20°C: about 65% of maximal activity, 60°C: about 75% of maximal activity, enzyme ecapsulated in ethyleneamine-mediated biosilica Acidovorax facilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, soluble enzyme loses 41% of its activity Acidovorax facilis
60
-
 2 h, soluble enzyme loses 82.5% of its activity. The enzyme ecapsulated in ethyleneamine-mediated biosilica retains 77.7% of its initial activity Acidovorax facilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 soluble and enzyme ecapsulated in ethyleneamine-mediated biosilica Acidovorax facilis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 65% of maximal activity, pH 10.0: about 75% of maximal activity, soluble enzyme Acidovorax facilis
6 10 pH 6.0: about 70% of maximal activity, pH 10.0: about 80% of maximal activity, enzyme ecapsulated in ethyleneamine-mediated biosilica Acidovorax facilis