Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 1.3fold stimulation | Fusarium proliferatum |
Cloned (Comment) | Organism |
---|---|
expression of the recombinant protein with His6-tag in Escherichia coli strain BL21 | Fusarium proliferatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, complete inhibition | Fusarium proliferatum | |
Ba2+ | 1 mM, 45% inhibition | Fusarium proliferatum | |
Co2+ | 1 mM, 35% inhibition | Fusarium proliferatum | |
Cu2+ | 1 mM, 30% inhibition | Fusarium proliferatum | |
Dichloromethane | 5% (v/v), 70% inhibition | Fusarium proliferatum | |
ethanol | 5% (v/v), 45% inhibition | Fusarium proliferatum | |
hexane | 5% (v/v), 80% inhibition | Fusarium proliferatum | |
Li+ | 1 mM, 20% inhibition | Fusarium proliferatum | |
methanol | 5% (v/v), 30% inhibition | Fusarium proliferatum | |
Ni2+ | 1 mM, 40% inhibition | Fusarium proliferatum | |
Propanol | 5% (v/v), 80% inhibition | Fusarium proliferatum | |
Toluene | 5% (v/v), 95% inhibition | Fusarium proliferatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.55 | - |
Benzonitrile | pH 8.0, 37°C | Fusarium proliferatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.1.5fold stimulation | Fusarium proliferatum | |
Fe2+ | 1 mM, 1.1fold stimulation | Fusarium proliferatum | |
Fe3+ | 1 mM, 1.25fold stimulation | Fusarium proliferatum | |
Mg2+ | 1 mM, 1.25fold stimulation | Fusarium proliferatum | |
Mn2+ | 1 mM, 1.2fold stimulation | Fusarium proliferatum | |
Zn2+ | 1 mM, 1.2fold stimulation | Fusarium proliferatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Fusarium proliferatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium proliferatum | A0A0E3D8K7 | - |
- |
Fusarium proliferatum AUF-2 | A0A0E3D8K7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusarium proliferatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.6 | - |
pH 8.0, 37°C, substrate: benzonitrile | Fusarium proliferatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-cyanopyridine + 2 H2O | 110% of the activity compared to benzonitrile | Fusarium proliferatum | pyridine 2-carboxylic acid + NH3 | - |
? | |
3-aminobutyronitrile + H2O | 201% of the activity compared to benzonitrile | Fusarium proliferatum | 3-aminobutyric acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | 151% of the activity compared to benzonitrile | Fusarium proliferatum | pyridine-3-carboxylic acid + NH3 | - |
? | |
3-nitrobenzonitrile + H2O | 110% of the activity compared to benzonitrile | Fusarium proliferatum | 3-nitrobenzoic acid + NH3 | - |
? | |
3-phenylpropionitrile + H2O | 151% of the activity compared to benzonitrile | Fusarium proliferatum | 3-phenylpropionate + NH3 | - |
? | |
4-aminobutyronitrile + H2O | 251% of the activity compared to benzonitrile | Fusarium proliferatum | 4-aminobutyric acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | 225% of the activity compared to benzonitrile | Fusarium proliferatum | pyridine-4-carboxylic acid + NH3 | - |
? | |
4-nitrobenzonitrile + H2O | 151% of the activity compared to benzonitrile | Fusarium proliferatum | 4-nitrobenzoic acid + NH3 | - |
? | |
acetonitrile + H2O | 135% of the activity compared to benzonitrile | Fusarium proliferatum | acetate + NH3 | - |
? | |
acetonitrile + H2O | 135% of the activity compared to benzonitrile | Fusarium proliferatum AUF-2 | acetate + NH3 | - |
? | |
acrylonitrile + H2O | 400% of the activity compared to benzonitrile | Fusarium proliferatum | acrylate + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Fusarium proliferatum | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Fusarium proliferatum AUF-2 | benzoic acid + NH3 | - |
? | |
butyronitrile + H2O | 425% of the activity compared to benzonitrile | Fusarium proliferatum | butyrate + NH3 | - |
? | |
butyronitrile + H2O | 425% of the activity compared to benzonitrile | Fusarium proliferatum AUF-2 | butyrate + NH3 | - |
? | |
di-phenylacetonitrile + H2O | 72% of the activity compared to benzonitrile | Fusarium proliferatum | ? + NH3 | - |
? | |
glutaronitrile + H2O | 319% of the activity compared to benzonitrile | Fusarium proliferatum | ? + NH3 | - |
? | |
mandelonitrile + H2O | 69% of the activity compared to benzonitrile | Fusarium proliferatum | ? + NH3 | - |
? | |
additional information | no activity with fumaronitrile, succinonitrile | Fusarium proliferatum | ? | - |
- |
|
additional information | no activity with fumaronitrile, succinonitrile | Fusarium proliferatum AUF-2 | ? | - |
- |
|
propionitrile + H2O | 416% of the activity compared to benzonitrile | Fusarium proliferatum | propionate + NH3 | - |
? | |
propionitrile + H2O | 416% of the activity compared to benzonitrile | Fusarium proliferatum AUF-2 | propionate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Fusarium proliferatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Fusarium proliferatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity | Fusarium proliferatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Fusarium proliferatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6.0: about 65% of maximal activity, pH 9.5: about 65% of maximal activity | Fusarium proliferatum |