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Literature summary for 3.5.5.1 extracted from

  • Dennett, G.V.; Blamey, J.M.
    A new thermophilic nitrilase from an antarctic hyperthermophilic microorganism (2016), Front. Bioeng. Biotechnol., 4, 5.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete loss of activity Pyrococcus sp. M24D13
dithiothreitol 5 mM, complete inhibition Pyrococcus sp. M24D13
glutathione 5 mM, complete inhibition Pyrococcus sp. M24D13
Hg2+ 5 mM, 98% loss of activity Pyrococcus sp. M24D13
iodoacetamide 5 mM, 82% loss of activity Pyrococcus sp. M24D13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
Benzonitrile pH 8.0, 80°C Pyrococcus sp. M24D13

Metals/Ions

Metals/Ions Comment Organism Structure
KCN 5 mM, 1.8fold activation, substrate: nenzonitrile Pyrococcus sp. M24D13

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
gel filtration Pyrococcus sp. M24D13

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. M24D13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus sp. M24D13

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3286.7
-
subatrate: benzonitrile, pH 8.0, 80°C Pyrococcus sp. M24D13

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + 2 H2O
-
Pyrococcus sp. M24D13 nicotinic acid + NH3
-
?
benzonitrile + 2 H2O
-
Pyrococcus sp. M24D13 benzoate + NH3
-
?
butyronitrile + H2O preference of the enzyme for phenylglycinonitrile Pyrococcus sp. M24D13 butyrate + NH3
-
?
mandelonitrile + 2 H2O
-
Pyrococcus sp. M24D13 mandelate + NH3
-
?
additional information no activity with molononitrile Pyrococcus sp. M24D13 ?
-
-
phenylglycinonitrile + H2O preference of the enzyme for phenylglycinonitrile and butyronitrile as substrates Pyrococcus sp. M24D13 phenylglycine + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Pyrococcus sp. M24D13

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Pyrococcus sp. M24D13

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
8 h, the enzyme retains more than 50% of its activity Pyrococcus sp. M24D13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pyrococcus sp. M24D13

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
205
-
Benzonitrile pH 8.0, 80°C Pyrococcus sp. M24D13