Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete loss of activity | Pyrococcus sp. M24D13 | |
dithiothreitol | 5 mM, complete inhibition | Pyrococcus sp. M24D13 | |
glutathione | 5 mM, complete inhibition | Pyrococcus sp. M24D13 | |
Hg2+ | 5 mM, 98% loss of activity | Pyrococcus sp. M24D13 | |
iodoacetamide | 5 mM, 82% loss of activity | Pyrococcus sp. M24D13 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Benzonitrile | pH 8.0, 80°C | Pyrococcus sp. M24D13 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCN | 5 mM, 1.8fold activation, substrate: nenzonitrile | Pyrococcus sp. M24D13 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
gel filtration | Pyrococcus sp. M24D13 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. M24D13 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. M24D13 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3286.7 | - |
subatrate: benzonitrile, pH 8.0, 80°C | Pyrococcus sp. M24D13 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | - |
Pyrococcus sp. M24D13 | nicotinic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Pyrococcus sp. M24D13 | benzoate + NH3 | - |
? | |
butyronitrile + H2O | preference of the enzyme for phenylglycinonitrile | Pyrococcus sp. M24D13 | butyrate + NH3 | - |
? | |
mandelonitrile + 2 H2O | - |
Pyrococcus sp. M24D13 | mandelate + NH3 | - |
? | |
additional information | no activity with molononitrile | Pyrococcus sp. M24D13 | ? | - |
- |
|
phenylglycinonitrile + H2O | preference of the enzyme for phenylglycinonitrile and butyronitrile as substrates | Pyrococcus sp. M24D13 | phenylglycine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Pyrococcus sp. M24D13 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Pyrococcus sp. M24D13 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
8 h, the enzyme retains more than 50% of its activity | Pyrococcus sp. M24D13 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pyrococcus sp. M24D13 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
205 | - |
Benzonitrile | pH 8.0, 80°C | Pyrococcus sp. M24D13 |