Literature summary for 3.5.5.1 extracted from

Woodward, J.D.; Trompetter, I.; Sewell, B.T.; Piotrowski, M.
Substrate specificity of plant nitrilase complexes is affected by their helical twist (2018), Commun. Biol., 1, 186 .

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Protein Variants

Protein Variants	Comment	Organism
C182A	inactive mutant	Capsella rubella
F80H	a single amino acid residue in position 80 (H80 in isoenzyme CrNIT1 and F80 in isoenzyme CrNIT2) is identified, that when exchanged, leads to an almost complete switch in substrate preference ratio. Position 80 exerts an influence on the helical twist	Capsella rubella
H80F	a single amino acid residue in position 80 (H80 in isoenzyme CrNIT1 and F80 in isoenzyme CrNIT2) is identified, that when exchanged, leads to an almost complete switch in substrate preference ratio. Position 80 exerts an influence on the helical twist	Capsella rubella
additional information	Helical twist and substrate size correlate and when binding pocket residues are exchanged between two nitrilases that show the same twist but different specificities, their specificities change	Capsella rubella

Organism

Organism	UniProt	Comment	Textmining
Capsella rubella	R0GJB9	-	-
Capsella rubella	R0H4W3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-butenenitrile + H2O	the enzyme displays a relatively high degree of specificity for 3-butenenitrile. Helical twist and substrate size correlate and when binding pocket residues are exchanged between two nitrilases that show the same twist but different specificities, their specificities change. It is proposed that helical twist influences the overall size of the binding pocket	Capsella rubella	3-butenoic acid + NH3	-	?
6-heptenenitrile + H2O	the enzyme shows a preference for several aliphatic alkenenitriles with peak activity against 6-heptenenitrile. Helical twist and substrate size correlate and when binding pocket residues are exchanged between two nitrilases that show the same twist but different specificities, their specificities change. It is proposed that helical twist influences the overall size of the binding pocket	Capsella rubella	6-heptenoic acid + NH3	-	?

Synonyms

Synonyms	Comment	Organism
CrNIT1	-	Capsella rubella
CrNIT2	-	Capsella rubella
nitrilase 1	-	Capsella rubella

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Release 2023.1 (January 2023)